Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Predio 43431, Sala 213, POBox 15005, Porto Alegre, RS, Brazil.
Biol Direct. 2013 Feb 12;8:6. doi: 10.1186/1745-6150-8-6.
tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.
tRNA 衍生的 RNA 片段(tRFs)是 19 个核苷酸的小 RNA,可与人类 Argonaute(AGO)蛋白结合。然而,在植物中,尚不清楚 tRFs 是否与 AGO 蛋白结合。在这里,我们使用免疫沉淀 Argonaute 蛋白(AGO-IP)的公共深度测序文库和生物信息学方法,鉴定了拟南芥 AGO-IP tRFs。此外,我们使用三个降解组深度测序文库,鉴定了四个假定的 tRF 靶标。还基于深度测序数据分析了 tRF 在非生物和生物胁迫下的表达模式。这里获得的结果为植物中 tRF 的进一步研究提供了有用的起点。