韧皮部汁液中的小RNA成分及其对缺铁的反应。
The Small RNA Component of Phloem Sap and Its Response to Iron Deficiency.
作者信息
Bakirbas Ahmet, Castro-Rodriguez Rosario, Walker Elsbeth L
机构信息
Biology Department and Plant Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, USA.
Biology Department, University of Massachusetts, Amherst, MA 01003, USA.
出版信息
Plants (Basel). 2023 Jul 27;12(15):2782. doi: 10.3390/plants12152782.
In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of , and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size-less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5' tRFs and 5' tRNA halves, are highly abundant in phloem sap, comprising about 46% of all reads. In addition, a set of miRNAs that are present in phloem sap was defined, and several miRNAs and sRNAs were identified that are differentially expressed during iron deficiency.
为了发现可能在缺铁胁迫期间发挥作用的小RNA（sRNA），从[植物名称]的韧皮部渗出液中提取RNA，并用于RNA测序。生物分析仪结果表明，来自韧皮部的丰富RNA尺寸较小，小于200个核苷酸。此外，未观察到典型的核糖体RNA（rRNA）条带。对八个独立的韧皮部RNA样本进行测序表明，源自转运RNA（tRNA）的片段，特别是5'端tRNA衍生片段（tRFs）和5'端tRNA半体，在韧皮部汁液中高度丰富，约占所有读数的46%。此外，还确定了一组存在于韧皮部汁液中的微小RNA（miRNA），并鉴定出了几种在缺铁期间差异表达的miRNA和sRNA。
Zotero 插件