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[轮状病毒感染诊断方法的比较:免疫荧光法、酶联免疫吸附测定法和乳胶凝集试验]

[Comparison of diagnostic methods in rotavirus infection: immunofluorescence, ELISA and the latex agglutination test].

作者信息

Arista S, Giovannelli L, Balsano M, Di Stefano R

出版信息

Quad Sclavo Diagn. 1984 Sep;20(3):258-63.

PMID:6099580
Abstract

To examine the sensitivity and reliability of a latex agglutination test for detecting rotavirus in faecal samples, we have tested 145 stool specimens from pediatric patients with or without diarrhea, comparing such latex test to immunofluorescent antibody technique (IF) and ELISA. Among all samples positive by IF and ELISA, 52.9% were positive by latex test. This percentage was raised to 85.7% when only positive samples from patients with diarrhea were considered. We conclude that the latex test is an useful diagnostic tool in acute diarrhea because of its simplicity and of its sufficient sensitivity.

摘要

为检测乳胶凝集试验在粪便样本中检测轮状病毒的敏感性和可靠性,我们对145份来自腹泻或未腹泻儿科患者的粪便标本进行了检测,并将该乳胶试验与免疫荧光抗体技术(IF)和酶联免疫吸附测定(ELISA)进行比较。在所有经IF和ELISA检测呈阳性的样本中,52.9%经乳胶试验呈阳性。当仅考虑腹泻患者的阳性样本时,这一比例升至85.7%。我们得出结论,乳胶试验因其操作简单且具有足够的敏感性,是急性腹泻中一种有用的诊断工具。

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Electropherotyping of human rotaviruses: an epidemiological survey of rotavirus infections in Sicily.人类轮状病毒的电泳分型:西西里岛轮状病毒感染的流行病学调查
Eur J Epidemiol. 1986 Jun;2(2):104-7. doi: 10.1007/BF00157019.