Xiang Wenqing, Peng Zhaoyang, Xu Jialu, Shen Hongqiang, Li Wei
Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou, China.
Department of Neurology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou, China.
J Clin Lab Anal. 2020 May;34(5):e23208. doi: 10.1002/jcla.23208. Epub 2020 Jan 13.
Rotavirus A and human adenovirus are the two most common causes of infantile diarrhea; thus, it is of great importance to find out a rapid and accurate diagnostic method. This study aimed to evaluate the diagnostic significance of latex agglutination test for detection of rotavirus A and human adenovirus.
A prospective study was conducted on 214 diarrhea children from September 2018 to March 2019 in our hospital. Fresh stool samples were collected for detection of rotavirus A and human adenovirus by latex agglutination test and quantitative reverse transcription polymerase chain reaction (RT-qPCR). Then, the consistency of results detected by these two methods was analyzed． RESULTS: With performing the latex agglutination test, it was revealed that positive rates for detecting rotavirus A virus and human adenovirus were 23.83% (51/214) and 25.24% (54/214), respectively. Meanwhile, results of RT-qPCR showed that positive rates for detecting rotavirus A virus and human adenovirus were 58 (27.10%) and 59 (27.57%), respectively. Using RT-qPCR as the gold standard, the sensitivity and specificity of the latex agglutination test for detecting rotavirus A were 81.03% and 97.44%, and the corresponding values for detecting human adenovirus were 76.27% and 94.19%, respectively.
This latex agglutination test showed a satisfactory consistency with RT-qPCR for detecting rotavirus A and human adenovirus. The mentioned commercial assay may be highly appropriate for rapid screening of rotavirus A and human adenovirus.
A组轮状病毒和人腺病毒是婴儿腹泻的两个最常见病因；因此，找出一种快速准确的诊断方法非常重要。本研究旨在评估乳胶凝集试验检测A组轮状病毒和人腺病毒的诊断意义。
2018年9月至2019年3月在我院对214例腹泻儿童进行了一项前瞻性研究。收集新鲜粪便样本，通过乳胶凝集试验和定量逆转录聚合酶链反应（RT-qPCR）检测A组轮状病毒和人腺病毒。然后，分析这两种方法检测结果的一致性。结果：进行乳胶凝集试验显示，检测A组轮状病毒和人腺病毒的阳性率分别为23.83%（51/214）和25.24%（54/214）。同时，RT-qPCR结果显示，检测A组轮状病毒和人腺病毒的阳性率分别为58例（27.10%）和59例（27.57%）。以RT-qPCR作为金标准，乳胶凝集试验检测A组轮状病毒的灵敏度和特异度分别为81.03%和97.44%，检测人腺病毒的相应值分别为76.27%和94.19%。
该乳胶凝集试验在检测A组轮状病毒和人腺病毒方面与RT-qPCR显示出令人满意的一致性。上述商业检测方法可能非常适合A组轮状病毒和人腺病毒的快速筛查。
