Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
J Cardiol. 2013 Mar;61(3):232-6. doi: 10.1016/j.jjcc.2012.12.003. Epub 2013 Feb 9.
Cholinergic nerve plays an important role in the induction and maintenance of atrial fibrillation (AF). Cholinergic innervation at supraventricular tissues is considered to be the histological basis and intervention-associated target site for the arrhythmia; however, the distribution of cholinergic nerve in supraventricular tissues has not been clearly studied. In this study, we investigated the cholinergic nerve innervation in canine supraventricular regions of hearts.
We performed histological and immunohistochemical staining on canine tissues of left atrial appendage (LAA), right atrial appendage (RAA), left atrium (LA), right atrium (RA), atrial septum (AS), crista terminalis (CT), pulmonary vein (PV), and super vena cava (SVC) using hematoxylin and eosin (H&E) and antibodies to choline acetyltransferase.
Normal canine cardiovascular histological structures were shown from H&E staining. Cholinergic nerve densities at LAA and RAA were significantly higher than LA, which was higher than RA, but no significant difference was observed between LAA and RAA. Furthermore, RA was significantly higher than AS, CT, PV, and SVC and there were no significant differences among the latter four.
The heterogeneity of different densities of cholinergic nerve innervation of canine supraventricular regions establishes the histological basis of cholinergic nerve-mediated pathological conditions.
胆碱能神经在心房颤动（AF）的发生和维持中起着重要作用。认为心腔的上腔组织中的胆碱能神经支配是心律失常的组织学基础和干预相关靶点；然而，上腔组织中的胆碱能神经分布尚未得到明确研究。在这项研究中，我们研究了犬上腔组织中的胆碱能神经支配。
我们使用苏木精和伊红（H&E）和胆碱乙酰转移酶抗体对犬的左心耳（LAA）、右心耳（RAA）、左心房（LA）、右心房（RA）、房间隔（AS）、终嵴（CT）、肺静脉（PV）和上腔静脉（SVC）组织进行了组织学和免疫组织化学染色。
H&E 染色显示了正常的犬心血管组织学结构。LAA 和 RAA 的胆碱能神经密度明显高于 LA，LA 又高于 RA，但 LAA 和 RAA 之间无显著差异。此外，RA 明显高于 AS、CT、PV 和 SVC，后四者之间无显著差异。
犬上腔组织中不同密度的胆碱能神经支配的异质性为胆碱能神经介导的病理条件奠定了组织学基础。
