Feng Ying-Cai, Liao Chong-Yu, Xia Wen-Kai, Jiang Xuan-Zhao, Shang Feng, Yuan Guo-Rui, Wang Jin-Jun
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, People's Republic of China.
Exp Appl Acarol. 2015 Sep;67(1):49-63. doi: 10.1007/s10493-015-9930-3. Epub 2015 Jun 11.
Superoxide dismutase (SOD) is a family of enzymes with multiple isoforms that possess antioxidative abilities in response to environmental stresses. Panonychus citri is one of the most important pest mites and has a global distribution. In this study, three distinct isoforms of SOD were cloned from P. citri and identified as cytoplasmic Cu-ZnSOD (PcSOD1), extracellular Cu-ZnSOD (PcSOD2), and mitochondrial MnSOD (PcSOD3). mRNA expression level analysis showed that all three isoforms were up-regulated significantly after exposure to the acaricide abamectin and to UV-B ultraviolet irradiation. In particular, PcSOD3 was up-regulated under almost all environmental stresses tested. The fold change of PcSOD3 expression was significantly higher than those of the two Cu-ZnSOD isoforms. Taken together, the results indicate that abamectin and UV-B can induce transcripts of all three SOD isoforms in P. citri. Furthermore, PcSOD3 seems to play a more important role in P. citri tolerance to oxidative stress.
超氧化物歧化酶（SOD）是一类具有多种同工型的酶，在应对环境胁迫时具有抗氧化能力。柑橘全爪螨是最重要的害虫螨之一，分布于全球。在本研究中，从柑橘全爪螨中克隆出三种不同的SOD同工型，并鉴定为细胞质铜锌超氧化物歧化酶（PcSOD1）、细胞外铜锌超氧化物歧化酶（PcSOD2）和线粒体锰超氧化物歧化酶（PcSOD3）。mRNA表达水平分析表明，在接触杀螨剂阿维菌素和UV-B紫外线照射后，所有三种同工型均显著上调。特别是，PcSOD3在几乎所有测试的环境胁迫下均上调。PcSOD3表达的倍数变化显著高于两种铜锌超氧化物歧化酶同工型。综上所述，结果表明阿维菌素和UV-B可诱导柑橘全爪螨中所有三种SOD同工型的转录本。此外，PcSOD3似乎在柑橘全爪螨对氧化应激的耐受性中发挥更重要的作用。
