基于单壁碳纳米管和结构转换DNA的无标记汞（II）化学电阻生物传感器。
Label-free chemiresistive biosensor for mercury (II) based on single-walled carbon nanotubes and structure-switching DNA.
作者信息
Gong Ji-Lai, Sarkar Tapan, Badhulika Sushmee, Mulchandani Ashok
机构信息
Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA ; College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
出版信息
Appl Phys Lett. 2013 Jan 7;102(1):13701. doi: 10.1063/1.4773569. Epub 2013 Jan 8.
Abstract
Herein, we present a sensitive, selective, and facile label-free DNA functionalized single-walled carbon nanotube (SWNT)-based chemiresistive biosensor for the detection of Hg(2+). SWNTs were functionalized with Hg(2+) binding 15-bases long polyT oligonucleotide through covalent attachment using a bilinker molecule. The polyT was further hybridized with polyA to form a polyT-polyA duplex. When exposed to Hg(2+) the polyT-polyA duplex was dehybridized combined with switching of polyT structure, leading to change in resistance/conductance of the SWNT chemiresistor device. The device provided a significant response within 100 to 1000 nM of Hg(2+) concentration with a 6.72 × 10(-3) nM(-1) sensitivity.
摘要
在此，我们展示了一种灵敏、选择性好且简便的基于无标记DNA功能化单壁碳纳米管（SWNT）的化学电阻型生物传感器，用于检测Hg(2+)。通过使用双连接分子共价连接，用与Hg(2+)结合的15个碱基长的聚胸腺嘧啶寡核苷酸对单壁碳纳米管进行功能化。聚胸腺嘧啶进一步与聚腺嘌呤杂交形成聚胸腺嘧啶-聚腺嘌呤双链体。当暴露于Hg(2+)时，聚胸腺嘧啶-聚腺嘌呤双链体去杂交，同时聚胸腺嘧啶结构发生转变，导致SWNT化学电阻器装置的电阻/电导发生变化。该装置在Hg(2+)浓度为100至1000 nM范围内有显著响应，灵敏度为6.72×10(-3) nM(-1)。
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