Centre of Bioanalysis, National Institute for Biological Sciences, Bucharest, Romania.
J Fluoresc. 2013 May;23(3):381-6. doi: 10.1007/s10895-013-1173-2. Epub 2013 Feb 14.
The natural methyl donor group, S-adenosylmethionine and its product, S-adenosylhomocysteine play an important role in many biochemical reactions involving transmethylation reactions. These compounds can be used as biomarkers in incipient diagnosis of various pathological disorders therefore the validation of a suitable method to routinely analysis of these compounds is very important. In this paper, a high performance liquid chromatrography method for S-adenosylmethionine and S-adenosylhomocysteine measurement as fluorescent 1,N(6)-ethanoderivatives from biological samples was validated in terms of selectivity, linearity range of the response (R > 0.9993), detection limit (9 × 10(-9) and 4.4 × 10(-9) mol L(-1)), the limit of quantitation (9.7 × 10(-9) and 5.7 × 10(-9) mol L(-1)), precision, trueness and robustness. The method for quantification simultaneous of these compounds is rapid, sensitive and precise and appropriate for clinical analysis.
天然甲基供体 S-腺苷甲硫氨酸及其产物 S-腺苷同型半胱氨酸在涉及转甲基反应的许多生化反应中发挥重要作用。这些化合物可用作各种病理紊乱初期诊断的生物标志物，因此验证一种合适的方法来常规分析这些化合物非常重要。本文建立了一种高效液相色谱法，通过对生物样品中 S-腺苷甲硫氨酸和 S-腺苷同型半胱氨酸进行荧光 1，N（6）-乙烷衍生化，以选择性、响应的线性范围（R＞0.9993）、检测限（9×10(-9)和 4.4×10(-9)mol/L(-1))、定量限（9.7×10(-9)和 5.7×10(-9)mol/L(-1))、精密度、准确度和稳健性进行了验证。该同时定量分析这些化合物的方法快速、灵敏、准确，适用于临床分析。
