Zwadlo-Klarwasser G, Bent S, Haubeck H D, Sorg C, Schmutzler W
Institute of Pharmacology, RWTH Aachen, FRG.
Int Arch Allergy Appl Immunol. 1990;91(2):175-80. doi: 10.1159/000235111.
The effect of intravenous administration of the glucocorticoid prednylidene on the macrophage subtype RM 3/1 in the peripheral blood of man was studied. Injection of 60 mg steroid resulted in an increase in the proportion of RM 3/1 positive monocytes 12 h after application from the basic level to about 80%. After 24 h the number of RM 3/1 positive cells decreased but remained elevated over the basis rate for a period of at least 15 days. Similar results were obtained after administration of 30 or 6 mg prednylidene even if the peak value occurred with a delay of 12 h after 6 mg. A dose- and time-dependent induction of the RM 3/1 positive subtype could also be demonstrated in vitro by the addition of prednylidene or dexamethasone to cultured purified human monocytes/macrophages. Deoxycortone or indomethacin had no effects. These results suggest that glucocorticoids exert their influence on cells of the mononuclear phagocytic lineage by inducing a distinct monocyte/macrophage subpopulation which seems to be associated with anti-inflammatory functions.
研究了静脉注射糖皮质激素泼尼松龙对人外周血中巨噬细胞亚型RM 3/1的影响。注射60mg类固醇后，给药12小时后，RM 3/1阳性单核细胞的比例从基线水平增加到约80%。24小时后，RM 3/1阳性细胞数量减少，但在至少15天的时间内仍高于基础水平。给予30mg或6mg泼尼松龙后也获得了类似的结果，即使6mg给药后峰值出现延迟12小时。通过向培养的纯化人单核细胞/巨噬细胞中添加泼尼松龙或地塞米松，在体外也可证明RM 3/1阳性亚型的剂量和时间依赖性诱导。脱氧皮质酮或吲哚美辛没有作用。这些结果表明，糖皮质激素通过诱导一个明显的单核细胞/巨噬细胞亚群对单核吞噬细胞系细胞发挥作用，该亚群似乎与抗炎功能相关。
