犬源PYY的结构表征

Structural characterization of canine PYY.

作者信息

Eysselein V E, Eberlein G A, Grandt D, Schaeffer M, Zehres B, Behn U, Schaefer D, Goebell H, Davis M, Lee T D

机构信息

Harbor-UCLA Medical Center, Department of Gastroenterology, Torrance 90509.

出版信息

Peptides. 1990 Jan-Feb;11(1):111-6. doi: 10.1016/0196-9781(90)90118-o.

DOI:10.1016/0196-9781(90)90118-o

PMID:2342986

Abstract

PYY was purified from canine colonic mucosa by sequential steps of reverse phase HPLC and ion-exchange FPLC. Microsequence, amino acid and mass spectral analyses of the purified peptide and its tryptic fragments were consistent with the structure: YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-amide. Canine PYY(1-36) has the identical sequence as porcine and rat PYY but differs from human PYY at position 3, with Ala instead of Ile, and position 18, with Ser instead of Asn. A smaller form, PYY(3-36), was also purified and characterized. It may differ in its biological activity from the intact peptide and could act as a partial antagonist or agonist of PYY(1-36).

摘要

通过反相高效液相色谱（HPLC）和离子交换快速蛋白质液相色谱（FPLC）的连续步骤，从犬结肠黏膜中纯化出肽YY（PYY）。对纯化后的肽及其胰蛋白酶片段进行微序列分析、氨基酸分析和质谱分析，结果与以下结构一致：YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-酰胺。犬PYY(1-36)与猪和大鼠的PYY序列相同，但在第3位与人类PYY不同，犬为丙氨酸（Ala）而非异亮氨酸（Ile），在第18位也不同，犬为丝氨酸（Ser）而非天冬酰胺（Asn）。还纯化并鉴定了一种较小的形式，即PYY(3-36)。它的生物活性可能与完整肽不同，并且可能作为PYY(1-36)的部分拮抗剂或激动剂发挥作用。