豚鼠α-新内啡肽的分离与微序列分析

Isolation and microsequence analysis of guinea pig alpha-neo-endorphin.

作者信息

Murphy R, Turner C A

机构信息

Department of Anatomy and Histology, School of Medicine, Flinders University, Bedford Park, Australia.

出版信息

Peptides. 1990 Jan-Feb;11(1):65-8. doi: 10.1016/0196-9781(90)90111-h.

DOI:10.1016/0196-9781(90)90111-h

PMID:2342991

Abstract

A multidimensional chromatographic regimen was used to isolate and purify alpha-neo-endorphin-like immunoreactive material from guinea pig small intestine. Microsequence analysis of the material obtained showed that the sequence of this peptide in guinea pig is the same as that previously reported for pig and rat, namely: H-Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys(OH). Thus, the sequence of alpha-neo-endorphin is conserved in all species thus far examined.

摘要

采用多维色谱方法从豚鼠小肠中分离并纯化α-新内啡肽样免疫反应性物质。对所得物质进行的微序列分析表明，该肽在豚鼠中的序列与先前报道的猪和大鼠的序列相同，即：H-酪氨酸-甘氨酸-甘氨酸-苯丙氨酸-亮氨酸-精氨酸-赖氨酸-酪氨酸-脯氨酸-赖氨酸（OH）。因此，α-新内啡肽的序列在迄今为止所研究的所有物种中都是保守的。