Shaw C, Thim L, Conlon J M
FEBS Lett. 1986 Jul 7;202(2):187-92. doi: 10.1016/0014-5793(86)80684-6.
Using three antisera to neurotensin of defined regional specificity, a novel neurotensin has been identified in extracts of guinea pig brain and small intestine. The primary structure of the peptide was established as: pGlu Leu Tyr Glu Asn Lys Ser Arg Arg Pro Tyr Ile Leu. Guinea pig neurotensin differs from bovine neurotensin by substitution of a prolyl residue by a seryl residue at position 7. Synthetic [Ser7]neurotensin showed identical chromatographic and immunochemical properties to guinea pig neurotensin. This difference in primary structure may account for some of the anomalous pharmacological effects of bovine neurotensin on guinea pig tissues.
利用三种具有特定区域特异性的抗神经降压素抗血清，在豚鼠脑和小肠提取物中鉴定出一种新型神经降压素。该肽的一级结构确定为：焦谷氨酸-亮氨酸-酪氨酸-谷氨酸-天冬酰胺-赖氨酸-丝氨酸-精氨酸-精氨酸-脯氨酸-酪氨酸-异亮氨酸-亮氨酸。豚鼠神经降压素与牛神经降压素的不同之处在于，在第7位脯氨酰残基被丝氨酰残基取代。合成的[丝氨酸7]神经降压素显示出与豚鼠神经降压素相同的色谱和免疫化学性质。一级结构的这种差异可能解释了牛神经降压素对豚鼠组织的一些异常药理作用。
