Bio-Iliberis Research and Development, I+D Department, 18210, Peligros, Granada, Spain.
Microb Biotechnol. 2013 May;6(3):307-13. doi: 10.1111/1751-7915.12037. Epub 2013 Feb 25.
Pseudomonas putida KT2440 has the ability to colonize the rhizosphere of a wide range of plants and can reach cell densities in the range of 10(5) -10(6) cfu g soil(-1) . Using the IVET technology we investigated which KT2440 genes were expressed in the rhizosphere of four different plants: pine, cypress, evergreen oak and rosemary. We identified 39 different transcriptional fusions containing the promoters of annotated genes that were preferentially expressed in the rhizosphere. Six of them were expressed in the rhizosphere of all the plant types tested, 11 were expressed in more than one plant and the remaining 22 fusions were found to be expressed in only one type of plant. Another 40 fusions were found to correspond to likely promoters that encode antisense RNAs of unknown function, some of which were isolated as fusions from the bacteria recovered in the rhizosphere from all of the plants, while others were specific to one or several of the plants. The results obtained in this study suggest that plant-specific signals are sensed by KT2440 in the rhizosphere and that the signals and consequent gene expression are related to the bacteria's successful establishment in this niche.
恶臭假单胞菌 KT2440 具有定殖广泛植物根际的能力，在根际中可达到 10(5) -10(6) cfu g 土壤(-1) 的细胞密度。我们使用 IVET 技术研究了 KT2440 在四种不同植物（松树、柏树、常绿橡树和迷迭香）根际中表达的哪些基因。我们鉴定了 39 个不同的转录融合体，其中包含注释基因的启动子，这些基因在根际中优先表达。其中 6 个在所有测试的植物类型的根际中表达，11 个在一种以上的植物中表达，其余 22 个融合体仅在一种植物中表达。另外 40 个融合体被发现对应可能的启动子，这些启动子编码未知功能的反义 RNA，其中一些是从所有植物根际中回收的细菌中分离出来的融合体，而另一些则是特定于一种或几种植物的融合体。本研究的结果表明，植物特异性信号被 KT2440 在根际中感知，信号和随之而来的基因表达与细菌在这一生态位中的成功建立有关。
