Ricardo Leyva Yanelys, Rodríguez Coto Maria Magdalena, Bisset Lazcano Juan A, Pérez Insueta Omayda, Sánchez Valdés Lizet
Departamento Control de Vectores, Instituto de Medicina Tropical Pedro Kouri, Ciudad da La Habana, Cuba.
Rev Cubana Med Trop. 2010 Sep-Dec;62(3):224-9.
the continuous use of the organophosphate temephos for Aedes (Stegomyia) aegypti (Linnaeus, 1762) has prompted the emergence of resistance to this product in several countries. Pyriproxyfen is an analogue of the juvenile hormone recommended by the World Health Organization as one of the alternative regulators of the dengue vector in drinking waters.
to evaluate the efficacy of pyriproxyfen in those strains with various degrees of temephos resistance, in order to be used as a possible regulator of Ae. aegypti.
for this study, two reference Ae. aegypti strains were used, one susceptible and the other resistant to temephos, and three field strains collected in Boyeros, Cotorro and 10 de Octubre municipalities, all of them from the City of Havana province, Cuba. For the temephos and pyriproxyfen evaluation, the WHO recommended bioassays were used.
at high concentrations, pyriproxyfen showed larvicidal properties. At 0,01 and 1 ppb concentrations, inhibition of emergence due to increase of pupal mortality and to lesser extent in adults in the process of breaking the pupal exubia was demonstrated. This growth regulator was effective at the same doses in all the strains, regardless of the degree of temephos resistance, being IE50 values within the range for Aedes genus.
according to the results, the effectiveness of pyriproxyfen was not affected by the degree of temephos resistance in the studied strains, and this is a useful tool in the Ae. aegypti control.
持续使用有机磷农药双硫磷来防治埃及伊蚊（林奈，1762年）已促使该产品在多个国家出现抗性。吡丙醚是一种保幼激素类似物，被世界卫生组织推荐为饮用水中登革热媒介的替代调节剂之一。
评估吡丙醚对不同程度双硫磷抗性品系的效果，以便用作埃及伊蚊可能的调节剂。
本研究使用了两个埃及伊蚊参考品系，一个对双硫磷敏感，另一个对双硫磷具有抗性，以及从古巴哈瓦那省博耶罗斯、科托罗和10月10日市收集的三个野外品系。对于双硫磷和吡丙醚评估，使用了世界卫生组织推荐的生物测定法。
在高浓度下，吡丙醚表现出杀幼虫特性。在0.01和1 ppb浓度下，由于蛹死亡率增加以及在破蛹过程中对成虫的影响较小，显示出羽化抑制作用。这种生长调节剂在所有品系中以相同剂量有效，无论双硫磷抗性程度如何，其IE50值在埃及伊蚊属范围内。
根据结果，吡丙醚的有效性不受所研究品系中双硫磷抗性程度的影响，这是埃及伊蚊防治中的一种有用工具。
