Universidade de Brasília, Programa de Pós-Graduação Stricto Sensu em Medicina Tropical, Brasília, DF, Brasil.
Ministério da Saúde, Secretaria de Vigilância em Saúde, Laboratório de Entomologia Médica, Brasília, DF, Brasil.
Rev Soc Bras Med Trop. 2020 Feb 7;53:e20190489. doi: 10.1590/0037-8682-0489-2019. eCollection 2020.
In Brasilia, pyriproxyfen (PPF; 0.01 mg/L) has been used for the larval control of Aedes aegypti mosquitoes since 2016. Information on the susceptibility of Ae. aegypti to PPF, and the development of resistance in populations from the Federal District of Brazil (FD) is limited. It is essential to monitor the susceptibility of Ae. aegypti to insecticides in order to improve vector control strategies. This study aimed to evaluate the susceptibility of Ae. aegypti populations from five areas of Brasilia to PPF.
We performed dose-response tests to estimate the emergence inhibition and resistance ratio of each field population, including the Rockefeller reference population. We also analyzed egg positivity, and the density and mortality of larvae and pupae.
Populations from Vila Planalto (RR50=1.7), Regiment Guards Cavalry (RR50=2.5), and Sub-secretary of Justice Complex (RR50=3.7) presented high susceptibility to PPF, while the RR values of populations from Lago Norte (RR50=7.7) and Varjão (RR50=5.9) were moderately high, suggesting the emergence of insipient resistance to PPF in Brasilia. At 30 ng/mL, the highest larvae mortality rate was 2.7% for the population from Lago Norte, while that of pupae was 92.1% for Varjão and Vila Planalto.
The five populations of Ae. aegypti from the FD are susceptible to PPF and there is a need to monitor the susceptibility of Ae. aegypti in new areas of the FD.
自 2016 年以来，巴西利亚一直在使用吡丙醚（PPF；0.01mg/L）控制埃及伊蚊的幼虫。有关埃及伊蚊对 PPF 的敏感性以及来自巴西联邦区（FD）的种群产生抗药性的信息有限。监测埃及伊蚊对杀虫剂的敏感性对于改进病媒控制策略至关重要。本研究旨在评估来自巴西利亚五个地区的埃及伊蚊种群对 PPF 的敏感性。
我们进行了剂量反应测试，以估计每个现场种群（包括洛克菲勒参考种群）的出蛹抑制率和抗性比。我们还分析了卵阳性率以及幼虫和蛹的密度和死亡率。
Vila Planalto（RR50=1.7）、Regiment Guards Cavalry（RR50=2.5）和 Sub-secretary of Justice Complex（RR50=3.7）的种群对 PPF 具有高敏感性，而 Lago Norte（RR50=7.7）和 Varjão（RR50=5.9）的种群的 RR 值则较高，表明巴西利亚对 PPF 出现了初始抗性。在 30ng/mL 时，Lago Norte 种群的幼虫死亡率最高为 2.7%，而 Varjão 和 Vila Planalto 种群的蛹死亡率则高达 92.1%。
来自 FD 的五种埃及伊蚊种群对 PPF 敏感，需要监测 FD 新地区的埃及伊蚊敏感性。
