Montgomery J A, Jetté M, Brunengraber H
Department of Nutrition, University of Montreal, Quebec, Canada.
Anal Biochem. 1990 Feb 15;185(1):71-6. doi: 10.1016/0003-2697(90)90256-9.
We present an assay for 2,3-butanediol by gas chromatography-mass spectrometry of its trimethylsilyl ethers. 2R,3R- and/or 2S,3S-2,3-butanediol and meso-2,3-butanediol are quantitated with corresponding internal standards of [2,3-2H2]butanediol. Limits of detection are 1 and 0.1 microM for split and splitless injections, respectively. Blood concentrations of 2,3-butanediol in nonalcoholics are 0.5 +/- 0.3 (SD) microM for 2R,3R- and/or 2S,3S-2,3-butanediol and 0.8 +/- 0.4 microM for meso-2,3-butanediol (n = 9). Two hours after alcohol ingestion, blood levels had risen in eight of nine subjects to 1.2 +/- 0.7 microM for 2R,3R-/2S,3S-2,3-butanediol and to 1.2 +/- 0.6 microM for meso-2,3-butanediol. Baseline urinary excretion of 2,3-butanediol is 0.4 +/- 0.2 mumol/mmol creatinine for 2R,3R-/2S,3S-2,3-butanediol and 0.9 +/- 0.5 mumol/mmol creatinine for meso-2,3-butanediol.
我们提出了一种通过对其三甲硅烷基醚进行气相色谱-质谱分析来测定2,3-丁二醇的方法。用[2,3-²H₂]丁二醇的相应内标对2R,3R-和/或2S,3S-2,3-丁二醇以及内消旋-2,3-丁二醇进行定量。对于分流进样和不分流进样，检测限分别为1微摩尔/升和0.1微摩尔/升。非酒精性受试者血液中2R,3R-和/或2S,3S-2,3-丁二醇的浓度为0.5±0.3（标准差）微摩尔/升，内消旋-2,3-丁二醇的浓度为0.8±0.4微摩尔/升（n = 9）。饮酒两小时后，9名受试者中有8名的血液水平上升，2R,3R-/2S,3S-2,3-丁二醇升至1.2±0.7微摩尔/升，内消旋-2,3-丁二醇升至1.2±0.6微摩尔/升。2R,3R-/2S,3S-2,3-丁二醇的2,3-丁二醇基线尿排泄量为0.4±0.2微摩尔/毫摩尔肌酐，内消旋-2,3-丁二醇为0.9±0.5微摩尔/毫摩尔肌酐。
