Jones K R, Rubin G M
Department of Molecular and Cell Biology, University of California-Berkeley 94720.
Neuron. 1990 May;4(5):711-23. doi: 10.1016/0896-6273(90)90197-n.
Mutation of the Drosophila melanogaster gene no-on-transient A (nonA) results in reduced visual acuity, behavior abnormalities, and an electrophysiological defect for which the mutant is named. We mapped the nonA gene genetically to a 20 kb interval within the 14C1,2 region of the X chromosome, isolated this chromosomal region, and used P element-mediated transformation to delimit the nonA gene to a 9 kb region. Analysis of cDNA clones indicates that this region encodes alternatively spliced transcripts encoding protein products of approximately 77 kd that differ only in their C-terminal 35 amino acids. Analysis of mutations generated in vitro in this transcription unit confirm that these transcripts are the products of the nonA gene.
果蝇黑腹基因无瞬时A（nonA）的突变会导致视敏度降低、行为异常以及一种电生理缺陷，该突变体也因此得名。我们通过遗传学方法将nonA基因定位到X染色体14C1,2区域内的一个20 kb区间，分离出该染色体区域，并利用P因子介导的转化将nonA基因限定在一个9 kb区域。对cDNA克隆的分析表明，该区域编码选择性剪接的转录本，这些转录本编码约77 kd的蛋白质产物，它们仅在C端的35个氨基酸上有所不同。对该转录单元体外产生的突变进行分析证实，这些转录本是nonA基因的产物。
