von Besser H, Schnabel P, Wieland C, Fritz E, Stanewsky R, Saumweber H
Institut für Entwicklungsphysiologie Universität zu Köln, Federal Republic of Germany.
Chromosoma. 1990 Dec;100(1):37-47. doi: 10.1007/BF00337601.
The DNA coding for the puff-specific protein Bj6 has been isolated by expression cloning. The gene is localized in 14C1,2 on the X chromosome and is expressed ubiquitously during embryonic development with prominent expression during the first 12 h of embryogenesis. cDNA and genomic clones have been sequenced and show a single open-reading frame of 2.1 kb length, coding for a Mr = 77,000 basic protein. In the aminoterminal half of the protein we detect stretches of repeated amino acids, centrally a region with homology to RNA-binding proteins containing the RNP 1 and RNP 2 consensus motif of RNA binding proteins, and the carboxyterminal part is rich in charged amino acids. The Bj6 protein is a product of the gene no-on transient A, a gene required for normal vision and courtship behaviour in Drosophila.
通过表达克隆分离出了编码胀泡特异性蛋白Bj6的DNA。该基因定位于X染色体的14C1,2区域，在胚胎发育过程中普遍表达，在胚胎发生的前12小时有显著表达。已对cDNA和基因组克隆进行了测序，显示有一个长度为2.1 kb的单一开放阅读框，编码一种分子量为77,000的碱性蛋白。在该蛋白的氨基末端一半区域，我们检测到重复氨基酸序列，中间有一个与含RNA结合蛋白的RNP 1和RNP 2共有基序的RNA结合蛋白具有同源性的区域，而羧基末端部分富含带电荷的氨基酸。Bj6蛋白是无瞬时A基因的产物，该基因是果蝇正常视觉和求偶行为所必需的。
