Polotskaia A V, Adler V V
Biokhimiia. 1990 Jan;55(1):102-8.
Glucocorticoids control protein synthesis and [3H]thymidine incorporation into DNA in resting and mitogen-stimulated in vitro human peripheral blood lymphocytes. This effect depends on the dose and time of hormone addition to the culture medium. Using two-dimensional electrophoresis, it was demonstrated that the steroids can regulate the expression of various proteins in intact and mitogen-stimulated human peripheral blood lymphocytes. This is suggestive of a dynamic control by glucocorticoids of the synthesized protein transcription during the cell cycle of a given population. These results also indicate that such a dynamic control is not due to the fluctuations in the number of receptor macromolecules, the presence or absence of the acceptor site on the DNA but, rather, to the modulation of the biological activity of specific nuclear factors capable of regulating the transcription of definite genes.
糖皮质激素可控制静息和有丝分裂原刺激的体外培养人外周血淋巴细胞中的蛋白质合成以及[3H]胸腺嘧啶核苷掺入DNA的过程。这种作用取决于激素添加到培养基中的剂量和时间。通过二维电泳证明，类固醇可调节完整的和有丝分裂原刺激的人外周血淋巴细胞中各种蛋白质的表达。这提示糖皮质激素在特定群体细胞周期中对合成蛋白转录进行动态调控。这些结果还表明，这种动态调控并非由于受体大分子数量的波动、DNA上受体位点的存在与否，而是由于能够调节特定基因转录的特定核因子生物活性的调节。
