[Regulation of proliferative and protein-synthesizing reactions in human peripheral blood lymphocytes by glucocorticoids].

Glucocorticoids control protein synthesis and [3H]thymidine incorporation into DNA in resting and mitogen-stimulated in vitro human peripheral blood lymphocytes. This effect depends on the dose and time of hormone addition to the culture medium. Using two-dimensional electrophoresis, it was demonstrated that the steroids can regulate the expression of various proteins in intact and mitogen-stimulated human peripheral blood lymphocytes. This is suggestive of a dynamic control by glucocorticoids of the synthesized protein transcription during the cell cycle of a given population. These results also indicate that such a dynamic control is not due to the fluctuations in the number of receptor macromolecules, the presence or absence of the acceptor site on the DNA but, rather, to the modulation of the biological activity of specific nuclear factors capable of regulating the transcription of definite genes.