分析和绘制源自人巨细胞病毒开放阅读框 UL30-UL32 的 3' 端共转录单元。

Analysis and mapping of a 3' coterminal transcription unit derived from human cytomegalovirus open reading frames UL30-UL32.

机构信息

Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.

出版信息

Virol J. 2013 Feb 27;10:65. doi: 10.1186/1743-422X-10-65.

DOI:10.1186/1743-422X-10-65

PMID:23446136

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3600006/

Abstract

BACKGROUND

It has been predicted that the UL31 gene originates from the positive strand of the human cytomegalovirus (HCMV) genome, whereas the UL30 and UL32 genes originate from the complementary strand. Except for the UL32 gene, the transcription of this gene region has not been investigated extensively.

METHODS

Northern blotting, cDNA library screening, RACE-PCR,and RT-PCR were used.

RESULTS

At least eight transcripts of the antisense orientation of UL31 were transcribed from the UL30-UL32 region during the late phase of HCMV infection. The 3' coterminus of these transcripts was located within the predicted UL30 gene. The longest 6.0-kb transcript was initiated upstream of the predicted UL32 gene. Other transcripts were derived from the predicted UL30 and UL31 gene region. Except for the previously predicted UL32 open reading frame (ORF), three novel ORFs, named UL31anti-1, UL31anti-2 and UL31anti-3, were located in the transcripts from the UL31anti-UL32 transcription unit. No transcription was found in UL31.

CONCLUSION

A family of novel 3' coterminal transcripts was transcribed from the UL30-UL32 gene region.

摘要

背景

据预测，UL31 基因来源于人巨细胞病毒（HCMV）基因组的正链，而 UL30 和 UL32 基因来源于互补链。除 UL32 基因外，该基因区域的转录尚未得到广泛研究。

方法

采用 Northern 印迹、cDNA 文库筛选、RACE-PCR 和 RT-PCR 等方法。

结果

在 HCMV 感染的晚期，至少有八种反义方向的 UL31 转录本从 UL30-UL32 区域转录。这些转录本的 3' 末端位于预测的 UL30 基因内。最长的 6.0kb 转录本起始于预测的 UL32 基因上游。其他转录本来自预测的 UL30 和 UL31 基因区域。除了先前预测的 UL32 开放阅读框（ORF）外，三个新的 ORF，命名为 UL31anti-1、UL31anti-2 和 UL31anti-3，位于 UL31anti-UL32 转录单元的转录本中。在 UL31 中没有发现转录。