Durmaz Bengul, Dalgalar Mehmet, Durmaz Riza
Faculty of Medicine, Department of Clinical Microbiology, Inonu University, 44069 Malatya, Turkey.
Anaerobe. 2005 Dec;11(6):318-21. doi: 10.1016/j.anaerobe.2005.06.001. Epub 2005 Jul 25.
In this age matched controlled study performed in Malatya, a city in east region of Turkey, enterotoxigenic Bacteroides fragilis (ETBF) was investigated in stool specimens obtained from children and adults with and without diarrhea. A nested polymerase chain reaction (PCR) method was used to detect the enterotoxin gene of B. fragilis in a total of 418 stool samples, including 221 samples from 117 children (aged 0-16 years) and 104 adults (aged >16 years) with diarrhea, and 197 samples from 102 children and 95 adults as control group that was the same age group with those having diarrhea. ETBF was detected in 13 of 117 diarrheal children (11.1%) and 8 of 102 control children (7.8%) (P>0.05). In children aged 1-5 years, the rate of ETBF was significantly higher in patients than in controls (25% versus 9.5%, respectively; P<0.05). On the other hand ETBF was detected similar rates (2.2% and 2.4%, respectively) in children younger than 1 year in both patients and controls. ETBF positivity was not significantly difference between patient and control groups who were older than 5 years of age and adults. The frequency of ETBF in the controls was slightly higher in older persons than in younger ones; however, it was not significant. The rate of ETBF as the only enteropathogen in the patients with ETBF was significantly higher than in controls with ETBF (88% versus 39%, respectively; P<0.02). We found that in east region of Turkey, the prevalence of ETBF was higher in the childhood diarrhea, particularly in aged 1-5. As the only enteropathogen, ETBF may play an important role in diarrheal diseases. Persons after 6 years old can be carrier for ETBF regardless diarrhea.
在土耳其东部城市马拉蒂亚进行的这项年龄匹配对照研究中，对有腹泻和无腹泻的儿童及成人粪便样本中的产肠毒素脆弱拟杆菌（ETBF）进行了调查。采用巢式聚合酶链反应（PCR）方法，在总共418份粪便样本中检测脆弱拟杆菌的肠毒素基因，其中包括来自117名腹泻儿童（0至16岁）和104名腹泻成人（大于16岁）的221份样本，以及来自102名儿童和95名成人的197份样本作为对照组，对照组与腹泻组为同一年龄组。117名腹泻儿童中有13名（11.1%）检测到ETBF，102名对照儿童中有8名（7.8%）检测到ETBF（P>0.05）。在1至5岁的儿童中，患者的ETBF检出率显著高于对照组（分别为25%和9.5%；P<0.05）。另一方面，1岁以下儿童患者和对照组中ETBF的检出率相似（分别为2.2%和2.4%）。5岁以上儿童及成人患者组和对照组之间ETBF阳性率无显著差异。对照组中ETBF的检出频率在老年人中略高于年轻人，但差异不显著。ETBF作为唯一肠道病原体在ETBF患者中的比例显著高于ETBF对照者（分别为88%和39%；P<0.02）。我们发现，在土耳其东部地区，儿童腹泻中ETBF的患病率较高，尤其是在1至5岁年龄段。作为唯一的肠道病原体，ETBF可能在腹泻疾病中起重要作用。6岁以后的人无论是否腹泻都可能是ETBF的携带者。
