Department of Cell Biology, Harvard Medical School, Boston, MA 02115-5701, USA.
Mol Biol Cell. 2013 Mar;24(5):535-42. doi: 10.1091/mbc.E12-09-0677.
A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.
对信号转导网络进行机理研究需要确定和分析磷酸化位点。质谱分析法是一种快速、高灵敏度的磷酸化位点作图方法。但是，在计划进行磷酸化位点作图时，必须考虑到质谱分析法的显著局限性。本文提供了在开始磷酸化位点分析之前应考虑的关键信息概述，以及成功进行实验的分步指南。
