Hilton C W, Reddy S, Prasad C, Wilber J F
Department of Medicine, Louisiana State University Medical Center, New Orleans 70112.
Endocr Res. 1990;16(2):139-50. doi: 10.1080/07435809009032995.
Changes in circulating cyclo(His-Pro) (CHP) levels after ingestion of 0.5 g/kg of glucose (oral) were measured in 32 Sprague Dawley rats at varying time points between 0 (before glucose) and 60 minutes. Each rat provided one time point. CHP levels displayed a biphasic response rising from 933 +/- 56 pg/ml to 1083 +/- 287 pg/ml (p greater than 0.05), followed by a fall to 543 +/- 46 pg/ml (p = 0.002) and then a recovery to near baseline values. To further investigate this response, a separate group of rats were sampled sequentially after 3 g/kg oral glucose (n = 7), 0.6 g/kg I.V. glucose (n = 6) or controls given saline only (n = 14). CHP levels were significantly elevated in the oral glucose group at 5 (p = 0.048) and 12 (p = 0.02) minutes compared to controls, while the i.v. glucose group was not statistically different from controls. Comparison of the mean of the highest incremental response to baseline values in each group revealed a greater excursion in CHP levels after oral (p = 0.027) glucose than after I.V. (p = 0.08) glucose. These data suggest CHP is acutely and reversibly elevated after glucose ingestion in rats and that the response is greater after oral glucose than after i.v. glucose.
在0（葡萄糖给药前）至60分钟的不同时间点，对32只Sprague Dawley大鼠口服0.5 g/kg葡萄糖后循环中环（组氨酸 - 脯氨酸）（CHP）水平的变化进行了测量。每只大鼠提供一个时间点的数据。CHP水平呈现双相反应，从933±56 pg/ml升至1083±287 pg/ml（p>0.05），随后降至543±46 pg/ml（p = 0.002），然后恢复至接近基线值。为进一步研究这种反应，另一组大鼠在口服3 g/kg葡萄糖（n = 7）、静脉注射0.6 g/kg葡萄糖（n = 6）或仅给予生理盐水的对照组（n = 14）后依次进行采样。与对照组相比，口服葡萄糖组在5分钟（p = 0.048）和12分钟（p = 0.02）时CHP水平显著升高，而静脉注射葡萄糖组与对照组无统计学差异。比较每组最高增量反应均值与基线值发现，口服葡萄糖（p = 0.027）后CHP水平的变化幅度大于静脉注射葡萄糖（p = 0.08）后。这些数据表明，大鼠摄入葡萄糖后CHP会急性且可逆地升高，并且口服葡萄糖后的反应大于静脉注射葡萄糖后的反应。
