Basu S, Chakrabarty A N, Ganguli M, Dastidar S G
Department of Medical Microbiology & Parasitology, University College of Medicine, Calcutta University.
Indian J Med Res. 1990 Mar;91:120-3.
Bacteriocinogeny was transferred at high frequencies from bacteriocinogenic (Bac+) V. cholerae strains to non-bacteriocinogenic (Bac-) recipients in the in situ genetic transformation system on agar surface. DNA extracted from samples of growth of bacteria transformed to Bac+ were obtained at 2 h intervals following contact with the sterile agar surface where the donor had grown previously. This showed acquisition of a high molecular weight plasmid which could be physically demonstrated best in the 6 h sample of the Bac+ transformants; their 4 h samples failed to show this specific plasmid, while it was demonstrable only as a faint band in the 8 h samples.
在琼脂表面的原位遗传转化系统中，产细菌素能力以高频率从产细菌素（Bac+）的霍乱弧菌菌株转移至不产细菌素（Bac-）的受体菌。在与供体菌先前生长过的无菌琼脂表面接触后，每隔2小时从转化为Bac+的细菌生长样本中提取DNA。这显示获得了一种高分子量质粒，在Bac+转化体的6小时样本中能最好地从物理上证明其存在；它们的4小时样本未显示出这种特定质粒，而在8小时样本中仅能作为一条 faint 带证明其存在。
