氧化苦参碱对D-半乳糖致衰老小鼠的影响

Zi Xiao-hu, Zhou Wei, Chen Qian, Li Mei, Gu Shu-ling

Department of Pharmacology, Xuzhou Medical College, Xuzhou 221004, China.

Zhong Yao Cai. 2012 Sep;35(9):1455-9.

OBJECTIVE

To investigate the effect of oxymatrine (OMT) on aging mouse caused by D (+)-Galactose.

METHODS

Healthy Kunming mouse were randomly divided into control, model and treated groups. The treated group was divided into three subgroups which were given oxymatrine 5 mg/kg, 10 mg/kg,20 mg/kg (OMT5, OMT10, OMT20). After 30 days of administration,the stand-jumping test and the Morris water maze test of each group were used to observe the learning and memory ability. After the behavior indicators test, 1-2 mL blood were got from ophthalmic vein plexus,and then got the serum by centrifuge. The super oxide dismutase (SOD) activity and malondialdehyde (MDA) content were determined by SOD and MDA kit.

RESULTS

In the water maze test, the model group was significantly longer in the latency than that of the control group (P < 0.01), and the number of the crossing platforms was significantly reduced (P < 0.01), but compared with the model group, the OMT5, OMT10, OMT20 groups were significantly shorter in the latency to the end (P < 0.05, P < 0.05, P < 0.01), and the number of the crossing platforms was significantly increased (P < 0.05, P < 0.05, P < 0.01); In the stand-jumping test,the OMT groups all could extend the platform latency and reduce the error reaction times, the learning ability of the OMT groups was better than the model group, but the statistical tests failed (P > 0.05); In the serum biochemical indicators test,the SOD activity of the model group was significantly lower than that of the control group (P < 0.01), but the SOD activity of OMT5, OMTIO, OMT20 groups was significant higher than that of the model group (P < 0.05, P < 0.05, P < 0.01); The MDA content of the model group was significantly higher than that of the control group (P < 0.01) but the MDA content of OMT5, OMT1O, OMT20 groups was significant lower than that of the model group (P < 0.05, P < 0.05, P < 0.01).

CONCLUSION

Oxymatrine could improve the learning and memory ability of aging mice caused by intraperitoneal injection D(+)-Galactose,and the anti-aging effect is related to the oxygen free radicals and reducing the lipid peroxidation.

目的

探讨氧化苦参碱（OMT）对D（+）-半乳糖致衰老小鼠的影响。

方法

将健康昆明小鼠随机分为对照组、模型组和治疗组。治疗组再分为三个亚组，分别给予氧化苦参碱5mg/kg、10mg/kg、20mg/kg（OMT5、OMT10、OMT20）。给药30天后，采用每组的直立跳跃试验和Morris水迷宫试验观察学习记忆能力。行为指标测试后，从眼静脉丛取血1-2mL，然后离心获得血清。采用超氧化物歧化酶（SOD）和丙二醛（MDA）试剂盒测定超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。

结果

在水迷宫试验中，模型组潜伏期明显长于对照组（P<0.01），穿越平台次数明显减少（P<0.01），但与模型组相比，OMT5、OMT10、OMT20组到达终点潜伏期明显缩短（P<0.05、P<0.05、P<0.01），穿越平台次数明显增加（P<0.05、P<0.05、P<0.01）；在直立跳跃试验中，OMT组均能延长平台潜伏期，减少错误反应次数，OMT组学习能力优于模型组，但统计学检验无差异（P>0.05）；在血清生化指标检测中，模型组SOD活性明显低于对照组（P<0.01），但OMT5、OMT10、OMT20组SOD活性明显高于模型组（P<0.05、P<0.05、P<0.01）；模型组MDA含量明显高于对照组（P<0.01），但OMT5、OMT10、OMT20组MDA含量明显低于模型组（P<0.05、P<0.05、P<0.01）。