Kim Min Jee, Choi Sei-Woong, Kim Iksoo
Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University , Gwangju 500-757 , Republic of Korea.
Mitochondrial DNA. 2013 Dec;24(6):622-4. doi: 10.3109/19401736.2013.772155. Epub 2013 Mar 1.
The larch hawk moth, Sphinx morio, belongs to the lepidopteran family Sphingidae that has long been studied as a family of model insects in a diverse field. In this study, we describe the complete mitochondrial genome (mitogenome) sequences of the species in terms of general genomic features and characteristic short repetitive sequences found in the A + T-rich region. The 15,299-bp-long genome consisted of a typical set of genes (13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes) and one major non-coding A + T-rich region, with the typical arrangement found in Lepidoptera. The 316-bp-long A + T-rich region located between srRNA and tRNA(Met) harbored the conserved sequence blocks that are typically found in lepidopteran insects. Additionally, the A + T-rich region of S. morio contained three characteristic repeat sequences that are rarely found in Lepidoptera: two identical 12-bp repeat, three identical 5-bp-long tandem repeat, and six nearly identical 5-6 bp long repeat sequences.
落叶松鹰蛾（Sphinx morio）属于鳞翅目天蛾科，长期以来该科一直作为一个模型昆虫家族在多个领域进行研究。在本研究中，我们从一般基因组特征以及富含A+T区域中发现的特征性短重复序列方面，描述了该物种的完整线粒体基因组（mitogenome）序列。这个长度为15299 bp的基因组由一组典型的基因（13个蛋白质编码基因、2个rRNA基因和22个tRNA基因）以及一个主要的非编码富含A+T区域组成，具有鳞翅目昆虫中常见的典型排列。位于srRNA和tRNA(Met)之间的长度为316 bp的富含A+T区域含有鳞翅目昆虫中通常发现的保守序列块。此外，落叶松鹰蛾的富含A+T区域包含三个在鳞翅目中很少见的特征性重复序列：两个相同的12 bp重复序列、三个相同的5 bp长串联重复序列以及六个几乎相同的5 - 6 bp长重复序列。
