Wang Ah Rha, Jeong Heon Cheon, Han Yeon Soo, Kim Iksoo
College of Agriculture & Life Sciences, Chonnam National University , Gwangju , Republic of Korea , and.
Mitochondrial DNA. 2014 Apr;25(2):93-4. doi: 10.3109/19401736.2013.784752. Epub 2013 Apr 16.
The mountainous duskywing, Erynnis montanus, belongs to a lepidopteran family Hesperiidae. The 15,530-bp long complete mitochondrial genome (mitogenome) of the species has the typical gene content of animals (13 protein-coding genes, two rRNA genes, 22 tRNA genes and one major non-coding A+T-rich region). As typical in lepidopteran mitogenome E. montanus mitogenome also contained a high A/T content in the whole genome (81.7%) and the CGA (arginine) as the start codon for the COI gene. Unlike other lepidopteran species, including two sequenced skippers, the E. montanus mitogenome has a unique arrangement tRNA(Ser)-tRNA(Asn), instead of the tRNA(Asn)-tRNA(Ser) found unanimously in other lepidopteran species, providing a new gene arrangement in Lepidoptera. Such rearrangement probably was likely caused by duplication of gene block tRNA(Ser)-tRNA(Asn) and subsequent random loss of tRNA(Asn) in the first copy and tRNA(Ser) in the second copy, resulting in the arrangement tRNA(Ser)-tRNA(Asn).
山地暗翅弄蝶（Erynnis montanus）属于鳞翅目弄蝶科。该物种15530碱基对长的完整线粒体基因组（线粒体基因组）具有动物典型的基因组成（13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个主要的非编码富含A+T区域）。如同鳞翅目线粒体基因组的典型情况一样，山地暗翅弄蝶的线粒体基因组在整个基因组中也含有较高的A/T含量（81.7%），并且以CGA（精氨酸）作为COI基因的起始密码子。与其他鳞翅目物种不同，包括两个已测序的弄蝶，山地暗翅弄蝶的线粒体基因组具有独特的tRNA(Ser)-tRNA(Asn)排列，而不是在其他鳞翅目物种中一致发现的tRNA(Asn)-tRNA(Ser)排列，这在鳞翅目中提供了一种新的基因排列方式。这种重排可能是由基因块tRNA(Ser)-tRNA(Asn)的重复以及随后第一拷贝中的tRNA(Asn)和第二拷贝中的tRNA(Ser)随机丢失导致的，从而产生了tRNA(Ser)-tRNA(Asn)的排列。
