Xu J K, Goodwin C S, Cooper M, Robinson J
Department of Microbiology, Royal Perth Hospital, Australia.
J Infect Dis. 1990 Jun;161(6):1302-4. doi: 10.1093/infdis/161.6.1302.
Tissue culture cells were exposed to supernatants of Helicobacter pylori for 24 h at 37 degrees C in the presence of various quantities of urea. In the normal human stomach the concentration of urea is less than or equal to 4 mmol/l, and in the presence of this low concentration up to 10% of Vero cells showed intracellular vacuolization. In the presence of 7.5 mmol/l urea, 25% of the cells showed vacuolization. With 30 mmol/l urea, the final pH was 7.6, indicating that vacuolization was not due to change of pH. The first report of vacuolization of tissue culture cells by H. pylori was in a system without added urea but with concentrated bacterial supernatant; 30% of H. pylori strains demonstrated a cytotoxic effect. In those experiments fetal calf serum was used; it contains 6 mmol/l urea but was used at a concentration of 10%. A urease inhibitor, acetohydroxamic acid, caused a 75% drop in the number of cells showing vacuolization, and ammonia caused vacuolization. Thus the urea of H. pylori probably causes this vacuolization.
将组织培养细胞在37摄氏度下，于存在不同量尿素的情况下，暴露于幽门螺杆菌的上清液中24小时。在正常人体胃中，尿素浓度小于或等于4毫摩尔/升，在此低浓度下，高达10%的Vero细胞出现细胞内空泡化。在存在7.5毫摩尔/升尿素的情况下，25%的细胞出现空泡化。在30毫摩尔/升尿素时，最终pH值为7.6，这表明空泡化并非由pH值变化引起。关于幽门螺杆菌导致组织培养细胞空泡化的首次报告是在一个未添加尿素但使用浓缩细菌上清液的系统中；30%的幽门螺杆菌菌株表现出细胞毒性作用。在那些实验中使用了胎牛血清；其含有6毫摩尔/升尿素，但使用浓度为10%。一种脲酶抑制剂，乙酰氧肟酸，使出现空泡化的细胞数量下降了75%，并且氨也导致了空泡化。因此，幽门螺杆菌的尿素可能导致了这种空泡化。
