TI Food and Nutrition, Nieuwe Kanaal 9A, 6709PA Wageningen, The Netherlands.
Carbohydr Res. 2013 Apr 5;370:67-71. doi: 10.1016/j.carres.2013.01.018. Epub 2013 Feb 1.
A tagF1-tagF2 deletion mutant of Lactobacillus plantarum lacks poly(glycerol phosphate) polymerase activity required for glycerol-type wall teichoic acid (WTA) biosynthesis. The mutant activates an alternative genetic locus, tarIJKL, encoding the enzymes for nucleotide activation and incorporation of ribitol in the WTA backbone polymer. This alternative ribitol-type WTA backbone and its repeating unit were isolated and characterized by HPAEC, UPLC-MS, NMR spectroscopy, and MALDI-TOF MS, using synthetic molecules as references. The structure was established as 1,5-linked poly(ribitol phosphate) which was substituted at the C-2 hydroxyl group of the ribitol residue with α-D-glucosyl at a frequency of 28%.
植物乳杆菌的 tagF1-tagF2 缺失突变体缺乏甘油型细胞壁磷壁酸（WTA）生物合成所需的多（甘油磷酸）聚合酶活性。该突变体激活了一个替代的遗传基因座 tarIJKL，该基因座编码 WTA 主链聚合物中核苷酸的激活和核糖醇掺入的酶。通过使用合成分子作为参考，通过 HPAEC、UPLC-MS、NMR 光谱和 MALDI-TOF MS，分离和表征了这种替代的核糖醇型 WTA 主链及其重复单元。该结构被确定为 1,5-连接的聚（核糖醇磷酸），其在核糖醇残基的 C-2 羟基上被 α-D-葡萄糖基取代，取代频率为 28%。
