Tomita Satoru, Irisawa Tomohiro, Tanaka Naoto, Nukada Tomoo, Satoh Eiichi, Uchimura Tai, Okada Sanae
Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Tokyo, Japan.
Biosci Biotechnol Biochem. 2010;74(5):928-33. doi: 10.1271/bbb.90736. Epub 2010 May 7.
The contents, components, and synthesis genes of cell wall teichoic acid (WTA) in 18 strains of Lactobacillus plantarum were compared. The WTA of each strain was classified by its components as being either the glycerol- or the ribitol-type. The different strains in the WTA type showed marked differences also in two gene regions, tagD1-tagF2 and lp_1816-tagB2, as for the presence or absence, nucleotide sequences, and transcriptional activities. Our results clearly showed that the tagD1-tagF2 and lp_1816-tagB2 regions contained the synthesis genes of the WTA backbone of L. plantarum. We verified that the genes in the tagD1-tagF2 region were involved in the synthesis of the glycerol-type backbone. Furthermore, we propose that the genes in the lp_1816-tagB2 region were tarI, tarJ, tarK, and tarL, which are involved in the synthesis of the ribitol-type backbone.
比较了18株植物乳杆菌细胞壁磷壁酸（WTA）的含量、组成成分和合成基因。根据各菌株WTA的组成成分，将其分为甘油型或核糖醇型。不同WTA类型的菌株在tagD1-tagF2和lp_1816-tagB2这两个基因区域，在存在与否、核苷酸序列和转录活性方面也表现出显著差异。我们的结果清楚地表明，tagD1-tagF2和lp_1816-tagB2区域包含植物乳杆菌WTA主链的合成基因。我们证实tagD1-tagF2区域的基因参与甘油型主链的合成。此外，我们提出lp_1816-tagB2区域的基因是tarI、tarJ、tarK和tarL，它们参与核糖醇型主链的合成。
