Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, UNICAMP, Campinas-SP, Brazil.
Life Sci. 2013 Apr 19;92(13):799-807. doi: 10.1016/j.lfs.2013.02.011. Epub 2013 Feb 27.
After undergoing lesions, tendons have disorganized collagen fibers compared to undamaged tendons. Arrabidaea chica leaves have the aglycones carajurin and carajurone, components of the antocyanins, with a strong pharmacological potential due to their healing properties. Thus, the aim of this study was to investigate the effect of topical application of A. chica extract during tendon healing.
The calcaneal tendon of Wistar rats was partially transected with subsequent treatment with A. chica extract (2.13 g/mL) followed by excision on the 7th, 14th and 21st days. Control rats received only saline treatment.
Transmission electron microscopy analysis showed the presence of a large amount of small segments of collagen fibrils in the transected region of the tendons on the 7th day in both the control and plant-treated groups. Considering the organization of the collagen fibers, higher values of birefringence were observed under polarization microscopy in the tendons of the plant-treated group on the 14th day compared to the control group. A larger quantity of dermatan sulfate was also detected after plant treatment in the same period. However, lesser dermatan and chondroitin sulfate were detected in the plant-treated group than in the control group on the 21st day. No differences were found in the values of birefringence between these groups. Intense metachromasy was observed in both transected groups on the 21st day.
In conclusion, the use of A. chica extract improves collagen organization and increases the quantity of dermatan sulfate on the 14th day of the tendon healing.
与未受损的肌腱相比，受损后的肌腱胶原纤维排列紊乱。Arrabidaea chica 的叶子含有糖苷 carajurin 和 carajurone，是花青素的成分，具有很强的药理学潜力，因为它们具有愈合特性。因此，本研究旨在探讨局部应用 A. chica 提取物对肌腱愈合的影响。
用 Wistar 大鼠跟腱部分横断，随后用 A. chica 提取物（2.13 g/mL）处理，第 7、14 和 21 天切除。对照组仅接受生理盐水处理。
透射电子显微镜分析显示，在第 7 天，两组的肌腱横断区都存在大量小的胶原纤维片段。考虑到胶原纤维的组织，在偏振显微镜下，植物处理组在第 14 天的胶原纤维各向异性值高于对照组。在同一时期，植物处理后还检测到更多的硫酸皮肤素。然而，在第 21 天，植物处理组的硫酸皮肤素和软骨素硫酸盐含量均低于对照组。这些组之间的各向异性值没有差异。在第 21 天，两组的横断区都观察到强烈的变色反应。
总之，A. chica 提取物的使用可以改善胶原纤维的组织，增加肌腱愈合第 14 天硫酸皮肤素的含量。
