School of Food and Nutritional Sciences, University College Cork, Cork, Ireland.
Life Sci. 2013 Apr 19;92(13):775-82. doi: 10.1016/j.lfs.2013.02.006. Epub 2013 Feb 20.
The potato glycoalkaloids, α-chaconine, α-solanine and solanidine, along with potato peel extracts were investigated for potential anti-inflammatory effects in vitro. Their potential to reduce two biomarkers of inflammation, cytokine and nitric oxide (NO) productions, were assessed in the stimulated Jurkat and macrophage models, respectively.
Cytokine and nitric oxide productions were stimulated in Jurkat and Raw 264.7 macrophages with Concanavalin A (Con A; 25 μg/ml) and lipopolysaccaride (LPS; 1 μg/ml), respectively. Selective concentrations of glycoalkaloids and potato peel extracts were added simultaneously with Con A or LPS for 24h to investigate their potential to reduce inflammatory activity.
α-Chaconine and solanidine significantly reduced interleukin-2 (IL-2) and interleukin-8 (IL-8) productions in Con A-induced Jurkat cells. The potato peel extracts did not influence cytokine production. In LPS-stimulated Raw macrophages, α-solanine, solanidine and two potato peel extracts significantly reduced induced NO production.
Our findings suggest that sub-cytotoxic concentrations of potato glycoalkaloids and potato peel extracts possess anti-inflammatory effects in vitro and with further investigation may be useful in the prevention of anti-inflammatory diseases.
研究了土豆糖苷生物碱α-茄碱、α-龙葵碱和茄啶，以及土豆皮提取物的体外抗炎作用。分别在刺激的 Jurkat 和巨噬细胞模型中评估了它们降低两种炎症生物标志物——细胞因子和一氧化氮（NO）产生的潜力。
用刀豆球蛋白 A（Con A；25μg/ml）和脂多糖（LPS；1μg/ml）分别刺激 Jurkat 和 Raw 264.7 巨噬细胞产生细胞因子和一氧化氮。同时加入选择浓度的糖苷生物碱和土豆皮提取物与 Con A 或 LPS 共孵育 24 小时，以研究它们降低炎症活性的潜力。
α-茄碱和茄啶显著降低了 Con A 诱导的 Jurkat 细胞中白细胞介素-2（IL-2）和白细胞介素-8（IL-8）的产生。土豆皮提取物不影响细胞因子的产生。在 LPS 刺激的 Raw 巨噬细胞中，α-龙葵碱、茄啶和两种土豆皮提取物显著降低了诱导的 NO 产生。
我们的研究结果表明，土豆糖苷生物碱和土豆皮提取物的亚细胞毒性浓度具有体外抗炎作用，进一步研究可能有助于预防抗炎性疾病。
