Radner H, Kleinert R, Vennigerholz F, Denk H
Institute of Pathology, University of Graz, School of Medicine, Austria.
Neuropathol Appl Neurobiol. 1990 Apr;16(2):171-7. doi: 10.1111/j.1365-2990.1990.tb00945.x.
A 50-year-old female patient died of an untreatable glioma apoplecticum. At autopsy a strongly vascularized glial tumour was found. The criteria for malignancy according to the WHO classification were only partially fulfilled by this tumour which displayed morphological features of an astrocytoma but could not be further subclassified. By light microscopy, angioma-like vascular proliferations, large cells with brightly eosinophilic cytoplasm, and small cells with hyperchromatic nuclei were found. Most large cells had vesicular, excentrically placed nuclei and contained fibrillary whorls or amorphous, irregular cytoplasmic inclusions. By immunohistochemical staining, using antibodies to glial fibrillary acidic protein (GFAP) the fibrillary whorls were identified as aggregates of glial filaments. The amorphous inclusions lacked GFAP immunoreactivity and appeared in the electron microscope as electron dense material surrounded by a dense network of glial filaments. The abnormal perikaryal inclusions of these atypical astrocytoma cells appeared to be peculiar alterations of Rosenthal fibres closely mimicing Mallory bodies.
一名50岁女性患者死于无法治疗的胶质瘤卒中。尸检发现一个血管高度丰富的胶质肿瘤。根据世界卫生组织分类，该肿瘤仅部分符合恶性标准，其显示出星形细胞瘤的形态特征，但无法进一步细分。通过光学显微镜检查，发现了血管瘤样血管增生、细胞质嗜酸性强的大细胞以及核染色质增多的小细胞。大多数大细胞有泡状、偏心的核，并含有纤维状漩涡或无定形、不规则的细胞质内含物。通过免疫组织化学染色，使用抗胶质纤维酸性蛋白（GFAP）抗体，纤维状漩涡被鉴定为胶质丝的聚集体。无定形内含物缺乏GFAP免疫反应性，在电子显微镜下表现为被密集的胶质丝网络包围的电子致密物质。这些非典型星形细胞瘤细胞异常的核周内含物似乎是紧密模仿马洛里小体的罗森塔尔纤维的特殊改变。
