Pannexin1 半通道对于 HIV 感染人源原代 CD4+T 淋巴细胞至关重要。
Pannexin1 hemichannels are critical for HIV infection of human primary CD4+ T lymphocytes.
机构信息
Departamento de Neurología, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
出版信息
J Leukoc Biol. 2013 Sep;94(3):399-407. doi: 10.1189/jlb.0512249. Epub 2013 Mar 1.
Abstract
HIV is a major public health issue, and infection of CD4(+) T lymphocytes is one of its key features. Whereas several cellular proteins have been identified that facilitate viral infection and replication, the role of hemichannels in these processes has not been fully characterized. We now show that the HIV isolates, R5 and X4, induced a transient-early (5-30 min) and a later, persistent (48-120 h) opening of Panx1 hemichannels, which was dependent on the binding of HIV to CD4 and CCR5/CXCR4 receptors. Blocking Panx1 hemichannels by reducing their opening or protein expression inhibited HIV replication in CD4(+) T lymphocytes. Thus, our findings demonstrate that Panx1 hemichannels play an essential role in HIV infection.
摘要
HIV 是一个主要的公共卫生问题，而 CD4(+)T 淋巴细胞的感染是其关键特征之一。虽然已经确定了几种促进病毒感染和复制的细胞蛋白，但这些过程中半通道的作用尚未完全阐明。我们现在表明，HIV 分离株 R5 和 X4 诱导 Panx1 半通道的短暂早期（5-30 分钟）和后期持续（48-120 小时）开放，这依赖于 HIV 与 CD4 和 CCR5/CXCR4 受体的结合。通过减少 Panx1 半通道的开放或蛋白表达来阻断 Panx1 半通道，可抑制 CD4(+)T 淋巴细胞中的 HIV 复制。因此，我们的发现表明 Panx1 半通道在 HIV 感染中发挥重要作用。
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