Dilley Andrew
Brighton and Sussex Medical School, Falmer, Brighton, UK.
Methods Mol Biol. 2013;982:213-25. doi: 10.1007/978-1-62703-308-4_14.
Chronic pain affects as many as one in five people. A proportion of patients with symptoms of neuropathic -pain do not have clinical signs of any obvious tissue or nerve injury. Such patients include those with diffuse limb pain, back pain, and complex regional pain syndrome type 1. These patients remain a clinical enigma. However, through the development of the neuritis model, it has become apparent that local nerve inflammation in the absence of gross pathology (i.e., axonal degeneration and demyelination) may underlie part of the mechanisms of pain. In this chapter, we describe a method to induce the neuritis model. We also describe in detail a reliable method to test for mechanical allodynia and heat hyperalgesia. Data that demonstrates the potential benefits of the neuroprotective agent ARA290 in reducing pain behavior in the neuritis model are presented.
慢性疼痛影响着多达五分之一的人群。一部分有神经性疼痛症状的患者没有任何明显组织或神经损伤的临床体征。这类患者包括那些患有弥漫性肢体疼痛、背痛和1型复杂性区域疼痛综合征的人。这些患者仍然是临床上的谜团。然而，通过神经炎模型的发展，很明显在没有明显病理学改变（即轴突变性和脱髓鞘）的情况下局部神经炎症可能是疼痛机制的一部分。在本章中，我们描述了一种诱导神经炎模型的方法。我们还详细描述了一种检测机械性异常性疼痛和热痛觉过敏的可靠方法。文中展示了神经保护剂ARA290在减轻神经炎模型疼痛行为方面潜在益处的数据。
