Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, India.
Can J Physiol Pharmacol. 2013 Feb;91(2):134-40. doi: 10.1139/cjpp-2012-0279. Epub 2013 Feb 15.
Lindane is a man-made organochlorine pesticide used for agricultural purposes. Since lindane-induced toxicity is mediated by free radical generation, this investigation was carried out to study the protective effects of gallic acid and quercetin against lindane-induced cardiotoxicity. Lindane (100 mg·(kg body mass)(-1)) was administered orally to rats for 30 days. Histological analysis revealed pathological changes in the heart of lindane-treated rats. Biochemical analysis of the lindane-treated animals showed elevated activity for serum marker enzymes, lipid peroxidation (LPO), and membrane-bound Ca(2+) ATPase, with a concomitant decrease in the level of non-enzymic antioxidant (GSH), enzymic antioxidants such as superoxide dismutase, catalase, glutathione peroxidase (GPx), and glutathione-S-transferase (GST), and membrane-bound ATPases like Na(+)/K(+) ATPase and Mg(2+) ATPase in heart tissue. The results suggest that gallic acid and quercetin offer protection against lindane-induced myocardial damage, possibly through maintaining levels of endogenous antioxidant enzymes and membrane bound ATPase activity, as well as inhibiting lipid peroxidation.
林丹是一种用于农业目的的人造有机氯杀虫剂。由于林丹诱导的毒性是通过自由基的产生介导的，因此进行了这项研究，以研究没食子酸和槲皮素对林丹诱导的心脏毒性的保护作用。林丹（100 mg·（kg 体重）（-1））经口给予大鼠 30 天。组织学分析显示林丹处理大鼠的心脏发生了病理变化。对林丹处理动物的生化分析表明，血清标记酶、脂质过氧化（LPO）和膜结合 Ca（2+）ATP 酶的活性升高，同时非酶抗氧化剂（GSH）、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽-S-转移酶（GST）等酶抗氧化剂以及 Na（+）/K（+）ATP 酶和 Mg（2+）ATP 酶等膜结合 ATP 酶的水平降低。结果表明，没食子酸和槲皮素提供了针对林丹诱导的心肌损伤的保护，可能是通过维持内源性抗氧化酶和膜结合 ATP 酶活性以及抑制脂质过氧化。
