Department of Biochemistry, University of Calcutta, Kolkata-700019, India.
J Agric Food Chem. 2013 Apr 10;61(14):3443-50. doi: 10.1021/jf3046686. Epub 2013 Mar 25.
The antioxidant property of the 70% aqueous ethanol extract of Phyllanthus amarus roots and its ether-soluble, ethyl acetate-soluble, and aqueous fractions were investigated by various in vitro assays. The root extracts showed higher DPPH, hydroxyl, superoxide, and nitric oxide radical scavenging and reducing power activity. Among all the samples, the ethyl acetate-soluble fraction demonstrated highest radical scavenging activity and total phenolics content. Twenty-eight different phenolic compounds were identified by LCMS/MS analysis of the ethyl acetate-soluble fraction. The majority of the compounds were found to exist as their glycosides, and many of these were gallic acid derivatives. Free epicatechin and gallic acid were also identified in the ethyl acetate-soluble fraction. The present investigation suggested that P. amarus root is a potent antioxidant and can be used for the prevention of diseases related to oxidative stress.
采用各种体外试验方法研究了叶下珠根的 70%乙醇水溶液提取物及其醚溶性、乙酸乙酯溶性和水相部分的抗氧化性能。根提取物表现出更高的 DPPH、羟基、超氧自由基和一氧化氮自由基清除能力和还原能力。在所有样品中，乙酸乙酯相表现出最高的自由基清除活性和总酚含量。通过 LCMS/MS 分析乙酸乙酯相，鉴定出 28 种不同的酚类化合物。大多数化合物被发现以糖苷的形式存在，其中许多是没食子酸衍生物。游离表儿茶素和没食子酸也在乙酸乙酯相部分中被鉴定出来。本研究表明，叶下珠根是一种有效的抗氧化剂，可用于预防与氧化应激相关的疾病。
