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八种被忽视水果提取物的抗氧化和α-葡萄糖苷酶抑制活性以及活性提取物的超高效液相色谱-串联质谱分析

Antioxidant and α-glucosidase inhibitory activities of eight neglected fruit extracts and UHPLC-MS/MS profile of the active extracts.

作者信息

Mohamed Yunus Siti Norhamimah, Abas Faridah, Jaafar Ahmad Haniff, Azizan Awanis, Zolkeflee Nur Khaleeda Zulaikha, Abd Ghafar Siti Zulaikha

机构信息

Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia.

Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia.

出版信息

Food Sci Biotechnol. 2021 Jan 6;30(2):195-208. doi: 10.1007/s10068-020-00856-x. eCollection 2021 Feb.

DOI:10.1007/s10068-020-00856-x
PMID:33732510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7914318/
Abstract

The 70% ethanolic extracts from eight neglected fruits; , , , , , , and were evaluated for their 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, α-glucosidase inhibitory activities as well as total phenolic content. The results of this study revealed that fruit extract demonstrated the most potent radical scavenging activity. Among the fruits examined for α-glucosidase inhibitory activity, and exhibited strong activity with no significant difference. The Pearson correlation indicated that the activities of and contributed by phenolic compounds. A total of 65 metabolites were tentatively identified by using ultra-high-performance liquid chromatography tandem mass spectrometry (UHLPC-MS/MS). These findings suggested that the possible application of and as a functional food with antioxidant and α-glucosidase inhibitory properties.

摘要

对8种被忽视水果(分别为……此处原文水果名称缺失……)的70%乙醇提取物进行了2,2-二苯基-1-苦基肼(DPPH)自由基清除能力、α-葡萄糖苷酶抑制活性以及总酚含量的评估。本研究结果表明,……此处原文水果名称缺失……水果提取物表现出最强的自由基清除活性。在检测α-葡萄糖苷酶抑制活性的水果中,……此处原文水果名称缺失……表现出较强活性且无显著差异。皮尔逊相关性表明,……此处原文水果名称缺失……的活性由酚类化合物所致。使用超高效液相色谱串联质谱法(UHLPC-MS/MS)初步鉴定出了总共65种代谢物。这些发现表明,……此处原文水果名称缺失……可能作为具有抗氧化和α-葡萄糖苷酶抑制特性的功能性食品。

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