使用呼出气冷凝物8-异前列腺素评估变应原吸入对轻度哮喘患者气道氧化应激的影响
Effect of allergen inhalation on airway oxidant stress, using exhaled breath condensate 8-isoprostane, in mild asthma.
作者信息
Sood Akshay, Qualls Clifford, Seagrave JeanClare, McDonald Jacob, Shohreh Rugia, Chiavaroli Annalisa, Schuyler Mark
机构信息
Department of Medicine, University of New Mexico Health Sciences Center School of Medicine, Albuquerque, NM, USA.
出版信息
J Asthma. 2013 Jun;50(5):449-56. doi: 10.3109/02770903.2013.780609. Epub 2013 Apr 10.
OBJECTIVE
Exhaled breath condensate (EBC) 8-isoprostane concentrations are increased in asthma, but it is not known if they acutely change following bronchoprovocation. The objective of this study was to evaluate EBC 8-isoprostane concentrations following allergen-induced bronchoprovocation in asthma.
METHODS
This comparison study included eight mild atopic asthmatics and six controls. Asthmatics were challenged with inhaled specific allergen, methacholine, and irrelevant allergen in random order. Controls were challenged with irrelevant allergen. EBCs collected at 0, 3, 6, 9, and 23 hours by the R-tube method were measured for 8-isoprostanes by ELISA technique. Repeated measures ANOVA technique was used for analysis.
RESULTS
EBC 8-isoprostane concentrations did not change following any inhalational challenge, as compared to baseline, in either asthmatics or controls.
CONCLUSIONS
EBC 8-isoprostane concentrations do not acutely change following bronchoprovocation in subjects with mild asthma.
目的
哮喘患者呼出气冷凝液（EBC）中8-异前列腺素浓度升高，但支气管激发试验后其是否会急性变化尚不清楚。本研究的目的是评估变应原诱发支气管激发试验后哮喘患者EBC中8-异前列腺素的浓度。
方法
本对照研究纳入了8名轻度特应性哮喘患者和6名对照者。哮喘患者随机依次接受吸入特异性变应原、乙酰甲胆碱和无关变应原激发试验。对照者接受无关变应原激发试验。采用R型管法在0、3、6、9和23小时收集EBC，用ELISA技术检测其中的8-异前列腺素。采用重复测量方差分析技术进行分析。
结果
与基线相比，哮喘患者和对照者在任何吸入激发试验后EBC中8-异前列腺素浓度均未发生变化。
结论
轻度哮喘患者支气管激发试验后EBC中8-异前列腺素浓度不会急性变化。
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