Project Directorate on Poultry, Rajendranagar, Hyderabad, India.
Poult Sci. 2013 Apr;92(4):910-5. doi: 10.3382/ps.2012-02736.
An experiment was carried out on myostatin gene with the objectives of identification of polymorphism in the myostatin gene and estimation of the effect of polymorphism on growth traits in chickens. Single-stranded conformation polymorphism followed by sequencing was performed to reveal polymorphism of the gene. A total of 13 haplotypes were observed across 3 chicken lines (PB-1 and CB as broiler lines and IWI as the layer line). Myostatin haplogroups had a significant effect on BW at 28, 42, and 49 d of age in the PB-1 line. The significant association of haplogroups was observed with BW at d 14 and 49 in the CB line. In the IWI layer line, the myostatin gene was polymorphic but had no significant association with growth traits. It is concluded that the myostatin gene was polymorphic and had a significant effect on growth traits in broiler chickens.
本研究旨在探究肌肉生长抑制素(myostatin)基因的多态性,并评估其对鸡生长性状的影响。通过单链构象多态性(Single-stranded conformation polymorphism,SSCP)分析结合测序,对该基因进行了多态性分析。在 3 个鸡品系(PB-1、CB 为肉鸡品系,IWI 为蛋鸡品系)中观察到了 13 种单倍型。在 PB-1 线中,肌肉生长抑制素单倍型对 28、42 和 49 日龄体重(BW)有显著影响。在 CB 线中,单倍型与 14 日龄和 49 日龄 BW 显著相关。在 IWI 蛋鸡线中,肌肉生长抑制素基因多态性与生长性状无显著关联。研究结果表明,肌肉生长抑制素基因在肉鸡中具有多态性,并对其生长性状有显著影响。