Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.
Int J Food Microbiol. 2013 Apr 15;163(1):6-13. doi: 10.1016/j.ijfoodmicro.2013.02.004. Epub 2013 Feb 16.
This study investigated the prevalence and characteristics of Campylobacteraceae including a range of fastidious species in porcine samples. Over a thirteen month period caecal contents (n=402) and pork carcass swabs (n=401) were collected from three pork abattoirs and pork products (n=399) were purchased at point of sale in the Republic of Ireland. Campylobacteraceae isolates were recovered by enrichment, membrane filtration and incubation in antibiotic free media under a modified atmosphere (3% O2, 5% H2, 10% CO2 and 82% N2). Campylobacteraceae isolates were identified as either genus Campylobacter or Arcobacter and then selected species were identified by Polymerase Chain Reaction (PCR). Campylobacteraceae were isolated from 103 (26%) caecal samples, 42 (10%) carcass swabs, and 59 (15%) pork products. Campylobacter coli was the most commonly isolated species found in (37%) all sample types but many fastidious species were also isolated including Campylobacter concisus (10%), Arcobacter butzleri (8%), Campylobacter helveticus (8%), Campylobacter mucosalis (6%), Arcobacter cryaerophilus (3%), Campylobacter fetus subsp. fetus (1%), Campylobacter jejuni subsp. jejuni (1%), Campylobacter lari (0.5%), Campylobacter curvus (0.5%) and Arcobacter skirrowii (0.5%). Among all isolates, 83% contained cadF and 98% flaA. In this study 35% of porcine C. coli were resistant to ciprofloxacin but none of the fastidious species demonstrated any resistance to this drug. The level of resistance to erythromycin was very high (up to 100%) in C. concisus and C. helveticus and this is a real concern as this is the current empiric drug of choice for treatment of severe gastroenteritic Campylobacter infections. The study shows that there is a much wider range of fastidious Campylobacteraceae present in porcine samples than previously assumed with C. concisus the second most common species isolated. The majority of fastidious Campylobacteraceae isolates obtained contained virulence genes and antibiotic resistance indicating potential public health significance.
本研究调查了包括一系列苛养种在内的弯曲菌科在猪样本中的流行率和特征。在 13 个月的时间里，从三个猪肉屠宰场采集盲肠内容物（n=402）和猪肉胴体拭子（n=401），并在爱尔兰共和国的销售点购买猪肉产品（n=399）。通过富集、膜过滤和在改良的大气（3%O2、5%H2、10%CO2 和 82%N2）下在无抗生素的培养基中孵育来回收弯曲菌科分离株。弯曲菌科分离株被鉴定为弯曲菌属或 Arcobacter 属，然后通过聚合酶链反应（PCR）选择特定种进行鉴定。弯曲菌科从 103（26%）个盲肠样本、42（10%）个胴体拭子和 59（15%）个猪肉产品中分离出来。在所有样本类型中，最常分离到的种是大肠杆菌，但也分离到了许多苛养种，包括 C. concisus（10%）、Arcobacter butzleri（8%）、Campylobacter helveticus（8%）、Campylobacter mucosalis（6%）、Arcobacter cryaerophilus（3%）、Campylobacter fetus subsp. fetus（1%）、Campylobacter jejuni subsp. jejuni（1%）、Campylobacter lari（0.5%）、Campylobacter curvus（0.5%）和 Arcobacter skirrowii（0.5%）。在所有分离株中，83%含有 cadF，98%含有 flaA。在本研究中，35%的猪大肠杆菌对环丙沙星有耐药性，但没有一种苛养种对这种药物表现出任何耐药性。在 C. concisus 和 C. helveticus 中，对红霉素的耐药性非常高（高达 100%），这是一个真正的问题，因为这是目前治疗严重胃肠道弯曲菌感染的经验性首选药物。研究表明，在猪样本中存在比以前认为的更广泛的苛养弯曲菌科，其中 C. concisus 是第二常见的分离种。获得的大多数苛养弯曲菌科分离株含有毒力基因和抗生素耐药性，表明具有潜在的公共卫生意义。
