Daewoong Pharmaceutical Co., Ltd, Seoul, Republic of Korea.
Hum Exp Toxicol. 2013 Sep;32(9):914-20. doi: 10.1177/0960327113479044. Epub 2013 Mar 8.
New cosmetic applications and products based on the effects of botulinum toxin (BTX) treatment have stimulated demand for this class of natural compounds. This demand generates the need for appropriate standardized protocols to test and compare the effectiveness of new BTX preparations.
Based on the previously described electrophysiological methods, we measured and compared the inhibitory effects of two BTX type A (BTX-A) preparations on neuromuscular transmission through split-body test.
The effectiveness was evaluated in terms of the compound muscle action potential (CMAP) and conduction velocity after BTX-A injection. We used a split-body method to compare two different BTX-As in the rat.
Based on the changes in the CMAP, the two different BTX-As induced paralytic effect on the rat tibialis anterior muscle. However, the two different BTX-A preparations did not differ significantly in effectiveness and did not induce a delay in conduction velocity.
The new BTX-A preparation used in this electrophysiological study had similar effect compared with the previously marketed BTX-A.[AQ: Please approve the edits made to the sentence "The new BTX-A preparation…") We propose that a split-body electrophysiological protocol will be useful in establishing the comparative effectiveness of new BTX products.
基于肉毒毒素 (BTX) 治疗效果的新型美容应用和产品刺激了对这类天然化合物的需求。这种需求产生了测试和比较新型 BTX 制剂有效性的适当标准化方案的需求。
基于先前描述的电生理学方法，我们通过分体测试测量和比较了两种 BTX 型 A（BTX-A）制剂对神经肌肉传递的抑制作用。
在 BTX-A 注射后，通过复合肌肉动作电位 (CMAP) 和传导速度来评估效果。我们使用分体方法在大鼠中比较了两种不同的 BTX-A。
基于 CMAP 的变化，两种不同的 BTX-A 引起了大鼠胫骨前肌的麻痹作用。然而，两种不同的 BTX-A 制剂在效果上没有显著差异，也没有引起传导速度的延迟。
本电生理学研究中使用的新型 BTX-A 制剂与先前上市的 BTX-A 相比具有相似的效果。我们建议分体电生理方案将有助于建立新型 BTX 产品的比较效果。
