通过硝酸银/硫酸铯密度梯度离心法对双游水霉（卵菌纲）卫星DNA进行分级分离。

Fractionation of Saprolegnia diclina (Oomycetes) satelite DNAs by AgNO3/Cs2SO4 density gradient centrifugation.

作者信息

Neish G A, Green B R

出版信息

Biochim Biophys Acta. 1977 Dec 14;479(4):411-5. doi: 10.1016/0005-2787(77)90034-x.

PMID:922010

Abstract

Saprolegnia diclina DNA has been fractionated using preparative AgNO3/Cs2SO4 and CsCl density gradients. In addition to the previously identified major satellite DNA, there are two minor DNA components banding at 1.682 and 1.701 g - cm(-3) in CsCl. Purified major satellite DNA bands at 1.707 g - cm(-3) giving a base composition of 48% G + C in good agreement with 47% G + C calculated from its Tm value. The nuclear DNA base composition is 58% G + C by both methods. The base composition of the major satellite DNA suggests that it may represent ribosomal DNA cistrons.

摘要

双游水霉DNA已通过制备性硝酸银/硫酸铯和氯化铯密度梯度进行分级分离。除了先前鉴定出的主要卫星DNA外，在氯化铯中还有两个次要DNA组分，其密度带分别为1.682和1.701 g - cm(-3)。纯化的主要卫星DNA密度带为1.707 g - cm(-3)，其碱基组成为48%的鸟嘌呤加胞嘧啶，这与根据其熔解温度计算出的47%的鸟嘌呤加胞嘧啶非常吻合。通过两种方法测定，核DNA的碱基组成为58%的鸟嘌呤加胞嘧啶。主要卫星DNA的碱基组成表明它可能代表核糖体DNA顺反子。