He Xi-Cheng, Shun Qin-Wen, Dong Li-Sha, Ge Xiang-Qian, Zhang Hua, Nong Heng, Shen Xiao-Hua
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3595-8.
Astilbin in 28 Smilax glabra (red and white cross-section) from different sources was determined by HPLC. Pharmacodynamics and component of S. glabra was investigated through inflammation experiment (penetration type).
The analysis was performed on a Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0. 1% acetic acid aqueous (21: 79) at a flow rate of 1.0 mL x min(-1). The detection wavelength was 291 nm, and the column temperature was 25 degrees C. Anti-inflammatory effect was compared from two type cross-section of Smilax glabra in capillary permeability experiment.
Linear correlation was good in the range of 0.003 379-4.004 microg, and the average recoveries were 100.1%, 101.9%, 99.3%, respectively. The content of astilbin in white and red transverse section were 0.19%-2.46% and 2.10%-5.92%, respectively. Anti-inflammatory efficiency of sectioned red and white were were 21% and 32%, respectively.
Astilbin content and anti-inflammatory effect is significantly different between red and white transverse section of S. glabra, the content of astilbin is not positively related with anti-inflammatory effect.
采用高效液相色谱法测定28份不同来源的光叶菝葜（红、白断面）中落新妇苷的含量。通过炎症实验（渗透型）研究光叶菝葜的药效学及成分。
采用Hypersil ODS2柱（4.6 mm×250 mm，5μm）进行分析，流动相为乙腈和0.1%醋酸水溶液（21∶79），流速为1.0 mL·min-1。检测波长为291 nm，柱温为25℃。在毛细血管通透性实验中比较光叶菝葜两种断面的抗炎作用。
在0.003 379 - 4.004μg范围内线性关系良好，平均回收率分别为100.1%、101.9%、99.3%。白色和红色横切面中落新妇苷的含量分别为0.19% - 2.46%和2.10% - 5.92%。红色和白色断面的抗炎效率分别为21%和32%。
光叶菝葜红、白横切面落新妇苷含量及抗炎作用存在显著差异，落新妇苷含量与抗炎作用无正相关关系。
