Wu Xin-Rong, Liu Zhi-Gang, Yan Ren-Liang, Sun Wei-Feng
Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Zhong Yao Cai. 2009 Oct;32(10):1606-9.
To investigate the process of separating and purifying total flavonoids from Smilax glabra.
With the contents of total flavonoids, astilbin and engelitin as indices, the optimum process of separating and purifying flavonoids from Smilax glabra was screened by static and dynamic adsorption tests.
The static saturated adsorption capacity of polyamide resin to flavonoids of Smilax glabra was 144 mg/g (saturated in 2 h). The dynamic adsorption capacity was 128.125 mg/g, the optimum conditions of elution were as follows: the adsorbed resin was washed by 20BV water firstly, then by 20BV 95% ethanol. The content of obtained total flavonoids reached 71.84%, as the content of astilbin was 14.32%, and the content of engelitin was 1. 08%.
The total flavonoids of Smilax glabra is able to be easily separated and purified by polyamide resin under the optimum conditions above.
研究从菝葜中分离纯化总黄酮的工艺。
以总黄酮、落新妇苷和恩贝素含量为指标，通过静态和动态吸附试验筛选从菝葜中分离纯化黄酮类化合物的最佳工艺。
聚酰胺树脂对菝葜黄酮类化合物的静态饱和吸附量为144 mg/g（2 h达到饱和）。动态吸附量为128.125 mg/g，最佳洗脱条件为：先用20BV水洗脱吸附的树脂，再用20BV 95%乙醇洗脱。得到的总黄酮含量达到71.84%，其中落新妇苷含量为14.32%，恩贝素含量为1.08%。
在上述最佳条件下，聚酰胺树脂能够轻松地从菝葜中分离纯化总黄酮。
