Sharma J M, Witter R L
Cancer Res. 1975 Mar;35(3):711-7.
Chickens were bursectomized by cyclophosphamide treatment at hatching. At 8 or 9 weeks of age, bursectomized and unbursectomized hatchmates, free from prior infection, were challenged with pathogenic Marek's disease virus. Oncogenicity of the virus inoculum was confirmed by inoculating 1-day-old susceptible chickens. At the time of virus challenge, blood cells from the cyclophosphamide-treated chickens were able to mount a vigorous graft-versus-host reaction in allogeneic embryos. This ability indicated that the thymus function was intact. There were no significant differences in Marek's disease response of bursectomized and unbursectomized chickens, in spite of a severe defect in the bursa-dependent functions in the bursectomized chickens. Some bursa-deficient chickens had non-proliferating, presumably regressing lesions in peripheral nerves. Because these lesions lacked plasma cells, it was concluded that the plasma cell may not play a functional role in recovery from Marek's disease.
雏鸡在孵化时通过环磷酰胺处理进行法氏囊切除。在8或9周龄时，对经过法氏囊切除和未经过法氏囊切除且无先前感染的同批孵化雏鸡，用致病性马立克氏病病毒进行攻击。通过接种1日龄易感雏鸡来确认病毒接种物的致癌性。在病毒攻击时，经环磷酰胺处理的雏鸡的血细胞能够在同种异体胚胎中引发强烈的移植物抗宿主反应。这种能力表明胸腺功能完好。尽管法氏囊切除的雏鸡在依赖法氏囊的功能方面存在严重缺陷，但法氏囊切除和未切除的雏鸡对马立克氏病的反应没有显著差异。一些法氏囊缺陷的雏鸡在外周神经中有不增殖、可能正在消退的病变。由于这些病变缺乏浆细胞，因此得出结论，浆细胞可能在马立克氏病的恢复中不发挥功能作用。
