Lu Y S, Kermani-Arab V, Moll T
Am J Vet Res. 1976 Jun;37(6):687-92.
Bursa- and thymus-dependent functions were examined in Marek's disease (MD)-susceptible normal chickens and in chickens treated with 5 and 16 mg of cyclophosphamide (CY) at the time of hatching. Chickens not exposed to Marek's disease virus (MDV) and treated with CY temporarily lost mitogenic response to concanavalin A but regained full response after 5 weeks. Bursa-dependent functions, such as presence of germinal centers in spleen and cecal tonsils, morphologic features of bursa, and sheep red blood cell antibody response were completely lost in chickens treated with 16 mg of CY and only partly retained in chickens treated with 5 mg of CY. In chickens exposed to MDV, the degree of thymus-dependent spleen cell mitogenic response was directly related to frequency and severity of MD. Chickens treated with 16 mg of CY had a mild mitogenic depression and low frequency and severity of MD lesions, whereas those treated with 5 mg of CY and those not treated had marked mitogenic depression and high frequency and severity of MD. Suppressions of bursa- and thymus-dependent functions by MDV alone were also evident when comparing MDV-exposed and nonexposed chickens. The results also indicate that presence of small, residual amounts of humoral factor(s) may enhance MDV oncogenesis.
对马立克氏病（MD）易感的正常鸡以及在孵化时用5毫克和16毫克环磷酰胺（CY）处理过的鸡，检测了其法氏囊和胸腺依赖性功能。未接触马立克氏病病毒（MDV）并用CY处理的鸡暂时失去了对刀豆球蛋白A的促有丝分裂反应，但在5周后恢复了完全反应。在用16毫克CY处理的鸡中，法氏囊依赖性功能，如脾脏和盲肠扁桃体中生发中心的存在、法氏囊的形态特征以及绵羊红细胞抗体反应完全丧失，而在用5毫克CY处理的鸡中仅部分保留。在接触MDV的鸡中，胸腺依赖性脾细胞促有丝分裂反应的程度与MD的发生率和严重程度直接相关。用16毫克CY处理的鸡有轻度的促有丝分裂抑制，MD病变的发生率和严重程度较低，而用5毫克CY处理的鸡和未处理的鸡有明显的促有丝分裂抑制，MD的发生率和严重程度较高。当比较接触MDV和未接触MDV的鸡时，单独由MDV引起的法氏囊和胸腺依赖性功能的抑制也很明显。结果还表明，少量残留体液因子的存在可能会增强MDV的致瘤作用。
