Amend D F, Smith L
Infect Immun. 1975 Jan;11(1):171-9. doi: 10.1128/iai.11.1.171-179.1975.
Infectious hematopoietic necrosis (IHN) is a rhabdoviral disease of rainbow trout (Salmo gairdneri). Trout were injected with IHNV, and various hematological and biochemical measurements of clinically ill fish were compared to uninfected controls. Infected fish had reduced corpuscular counts, hemoglobin, and packed cell volume, but normal mean corpuscular volume, mean corpuscular hemoglobulin, and mean corpuscular hemoglobin concentration. The percentage of immature erythrocytes was increased, but the percentage of leukocytes was unchanged. Differential leukocyte counts showed a significant decrease in neutrophils, increase in lymphocytes, but no change in monocytes. Unidentifiable necrobiotic cells were prevelant in blood smears and hematopoietic tissue imprints. Plasma bicarbonate, chloride, calcium, phosphorus, bilirubin, and osmolality were signigicantly reduced, but plasma glucose and anterior kidney ascorbate were unchanged. Plasma pH increased and the alpha fractions of the serum proteins were altered. No change was found in plasma enzymes, except that a LDH isozyme was significantly increased. The alkali reserve was diminished and alterations in acid-base and fluid balance occurred. Death probably resulted from a severe electrolyte and fluid imbalance caused by renal failure.
传染性造血器官坏死病（IHN）是虹鳟（Salmo gairdneri）的一种弹状病毒病。将虹鳟注射传染性造血器官坏死病毒（IHNV），并将临床患病鱼的各种血液学和生化指标与未感染的对照鱼进行比较。感染的鱼红细胞计数、血红蛋白和红细胞压积降低，但平均红细胞体积、平均红细胞血红蛋白含量和平均红细胞血红蛋白浓度正常。未成熟红细胞的百分比增加，但白细胞的百分比没有变化。白细胞分类计数显示中性粒细胞显著减少，淋巴细胞增加，单核细胞无变化。在血液涂片和造血组织印片中可见无法识别的坏死细胞。血浆碳酸氢盐、氯、钙、磷、胆红素和渗透压显著降低，但血浆葡萄糖和前肾抗坏血酸无变化。血浆pH值升高，血清蛋白的α组分发生改变。血浆酶未见变化，只是乳酸脱氢酶同工酶显著增加。碱储备减少，酸碱和体液平衡发生改变。死亡可能是由肾衰竭导致的严重电解质和体液失衡所致。
