Ghiasi Maryam, Binaii Mohammad, Ghasemi Mohdes, Fazli Hasan, Zorriehzahra Mohamad Jalil
Caspian Sea Ecology Research Center, P.O. Box 961, Farah-Abad, Sari, Iran.
Inland Water Aquaculture Institute, Anzali, Iran.
Virusdisease. 2016 Mar;27(1):12-8. doi: 10.1007/s13337-015-0289-1. Epub 2015 Nov 24.
Betanoda virus is an emerging problem in several marine fish species in various geographic areas all over the world. In recent years, mullets stock of the Caspian Sea decreased dramatically and a betanoda virus like-agent was introduced as the cause of mullet's mortality. The main objective of the present study is to compare hemato-biochemical parameters in healthy and infected sharpnose mullets (Liza saliens) to betanoda virus like-agent. The adult sharpnose mullets (34 clinically affected fish + 34 apparently healthy fish) were captured from the southeast the Caspian Sea from 2012 to 2013. All of the captured fish were 4 or 5 years old. The main clinical sings of infected fish were lethargy, severe abdominal distention, abnormal swimming and hyperinflation of swim bladder. The results showed that the weight and total length of infected fish were significantly lower than the healthy fish. The results of histopatological evaluation and indirect florescent antibody test were confirmed the presence of a betanoda virus-like agent in infected fish. The diseased fish were severely anemic (hypochromic macrocytic anemia) and had a serious haemopoietic disorders. The anemia associated with a sever leukopenia, a significant rise of neutrophils and immature neutrophils and a significant decries of lymphocytes percentage. Total protein, albumin and total immunoglobulin levels were significantly reduced in the serum of infected fish, while the activities of aspartate aminotransferase and alanine aminotransferase significantly increased when compared to the healthy fish. These results suggested that the feeding of the infected fish to betanoda virus like-agent were disturbed and it could be cause haemato-biochemical disorder and mortality of the fish in Iranian water of the Caspian Sea.
贝塔诺达病毒是世界各地多个地理区域几种海洋鱼类中出现的一个新问题。近年来，里海的鲻鱼种群数量急剧下降，一种类似贝塔诺达病毒的病原体被认定为鲻鱼死亡的原因。本研究的主要目的是比较健康和感染了类似贝塔诺达病毒病原体的尖吻鲻（Liza saliens）的血液生化参数。2012年至2013年，从里海东南部捕获了成年尖吻鲻（34条临床患病鱼 + 34条看似健康的鱼）。所有捕获的鱼均为4或5岁。感染鱼的主要临床症状为嗜睡、严重腹胀、异常游动和鱼鳔充气过度。结果表明，感染鱼的体重和全长显著低于健康鱼。组织病理学评估和间接荧光抗体试验结果证实感染鱼中存在类似贝塔诺达病毒的病原体。患病鱼严重贫血（低色素大细胞性贫血），并有严重的造血障碍。贫血伴有严重白细胞减少、中性粒细胞和未成熟中性粒细胞显著增多以及淋巴细胞百分比显著降低。与健康鱼相比，感染鱼血清中的总蛋白、白蛋白和总免疫球蛋白水平显著降低，而天冬氨酸转氨酶和丙氨酸转氨酶的活性显著升高。这些结果表明，感染了类似贝塔诺达病毒病原体的鱼的摄食受到干扰，这可能导致里海伊朗水域鱼类出现血液生化紊乱和死亡。
