Schneider P E, Miller H I, Nakamoto T
Department of Pediatric Dentistry, Louisiana State University Medical Center, School of Dentistry, New Orleans 70119.
Res Exp Med (Berl). 1990;190(2):131-6. doi: 10.1007/pl00020015.
The objective of this study was to evaluate the extended effect of caffeine intake received during gestation and lactation on the mandible and femur of rats. Timed-pregnant dams were divided into two groups. Dams of group 1 were fed a 20% protein diet throughout the experimental period from day 9 of gestation. Dams of group 2 were also fed a 20% protein diet, supplemented with caffeine (1 mg/100 g of body weight). Upon delivery, 8 pups were assigned to each dam, and the dams were continued on their respective diets. At weaning (day 22 postnatally), only male rats were selected. Pups of both groups were fed a 20% protein diet without caffeine. At day 56 postnatally the rats were killed. Mandibles and femurs were removed and the following parameters analyzed: weight, physical dimension, volume, and Knoop microhardness. Caffeine intake during gestation and lactation resulted in an impairment of femur growth and development and to a lesser extent mandibular growth and development. The early effects of caffeine in the maternal diet were lasting, as noted by the lack of recovery of the offspring even after changing to a caffeine-free diet for an extended time after weaning.
本研究的目的是评估妊娠期和哺乳期摄入咖啡因对大鼠下颌骨和股骨的长期影响。将定时受孕的母鼠分为两组。第1组母鼠在从妊娠第9天开始的整个实验期间喂食20%蛋白质饮食。第2组母鼠也喂食20%蛋白质饮食，并补充咖啡因（1毫克/100克体重）。分娩后，给每只母鼠分配8只幼崽，母鼠继续各自的饮食。在断奶时（出生后第22天），只选择雄性大鼠。两组幼崽均喂食不含咖啡因的20%蛋白质饮食。在出生后第56天处死大鼠。取出下颌骨和股骨，分析以下参数：重量、物理尺寸、体积和努氏显微硬度。妊娠期和哺乳期摄入咖啡因导致股骨生长发育受损，下颌骨生长发育受损程度较轻。母鼠饮食中咖啡因的早期影响是持久的，断奶后即使长时间改为无咖啡因饮食，后代也未恢复，这一点很明显。
